Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

WAPL (D9J1U) Rabbit mAb #77428

No. Size Price
77428S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
77428 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 160 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

WAPL (D9J1U) Rabbit mAb recognizes endogenous levels of total WAPL protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human WAPL protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using WAPL (D9J1U) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, transfected with control siRNA (-) or WAPL siRNA (+), using WAPL (D9J1U) Rabbit mAb (upper) or α-Actinin (D6F6) Rabbit mAb #6487 (lower).


The cohesin protein complex consists of a heterodimer between SMC1 (SMC1A or B) and SMC3, bound by additional RAD21 and STAG proteins (STAG1, 2, or 3). These proteins form a ring-like structure that mediates the cohesion of two sister chromatids after DNA replication in S phase (1,2). 

WAPL, also wings apart-like or WAPAL, is a chromatin-associated protein that binds to the cohesin protein complex and regulates its function and its interaction with chromatin (3,4). WAPL, through its interaction with cohesin, is important in the regulation of chromosome structure, cell cycle progression and appropriate chromosome segregation (5,6). Depletion of WAPL leads to segregation errors and DNA damage, and in p53 deficient cells leads to aneuploidy (6).

  1. Peters, J.M. et al. (2008) Genes Dev 22, 3089-114.
  2. Barbero, J.L. (2009) Cell Mol Life Sci 66, 2025-35.
  3. Kueng, S. et al. (2006) Cell 127, 955-67.
  4. Gandhi, R. et al. (2006) Curr Biol 16, 2406-17.
  5. Tedeschi, A. et al. (2013) Nature 501, 564-8.
  6. Haarhuis, J.H. et al. (2013) Curr Biol 23, 2071-7.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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