Product Pathways - Chromatin Regulation / Epigenetics
PHF19 Antibody #77271
|77271S||100 µl ( 10 western blots )||￥3,100.00 现货查询||购买询价|
|77271||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation,
Specificity / Sensitivity
PHF19 Antibody recognizes endogenous levels of total PHF19 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PHF19 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from K-562, COS-7, and DU 145 cell lines using PHF19 Antibody.
PHD finger protein 19 (PHF19), also known as polycomb-like protein 3 (PCL3), is a polycomb group protein that functions as an accessory subunit of the polycomb repressor complex 2 (PRC2), which represses target gene expression through methylation of histone H3 at lysine 27 by the EZH2 methyltransferase (1). PHF19 recruits PRC2 to target genes by binding trimethylated histone H3 lysine 36, a mark of active chromatin, via its Tudor domain (2-4). PHF19 associates with PRC2 and the histone H3 lysine 36 demethylases NO66 and FBXL10, and is required to recruit PRC2 and NO66/FBXL10 to stem cell genes during differentiation, resulting in PRC2-mediated trimethylation of histone H3 lysine 27, loss of trimethylated histone H3 lysine 36, and transcriptional silencing (2-4). Thus, PHF19 is critical for the proper transition of stem cell genes from the active to inactive state during differentiation of embryonic stem cells.
- Sauvageau, M. and Sauvageau, G. (2010) Cell Stem Cell 7, 299-313.
- Brien, G.L. et al. (2012) Nat Struct Mol Biol 19, 1273-81.
- Ballaré, C. et al. (2012) Nat Struct Mol Biol 19, 1257-65.
- Cai, L. et al. (2013) Mol Cell 49, 571-82.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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