Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

PHF19 Antibody #77271

No. Size Price
77271S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
77271 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 70 Rabbit
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

PHF19 Antibody recognizes endogenous levels of total PHF19 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PHF19 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from K-562, COS-7, and DU 145 cell lines using PHF19 Antibody.



Immunoprecipitation of PHF19 from K-562 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is PHF19 Antibody. Western blot analysis was performed using PHF19 Antibody.


PHD finger protein 19 (PHF19), also known as polycomb-like protein 3 (PCL3), is a polycomb group protein that functions as an accessory subunit of the polycomb repressor complex 2 (PRC2), which represses target gene expression through methylation of histone H3 at lysine 27 by the EZH2 methyltransferase (1). PHF19 recruits PRC2 to target genes by binding trimethylated histone H3 lysine 36, a mark of active chromatin, via its Tudor domain (2-4). PHF19 associates with PRC2 and the histone H3 lysine 36 demethylases NO66 and FBXL10, and is required to recruit PRC2 and NO66/FBXL10 to stem cell genes during differentiation, resulting in PRC2-mediated trimethylation of histone H3 lysine 27, loss of trimethylated histone H3 lysine 36, and transcriptional silencing (2-4). Thus, PHF19 is critical for the proper transition of stem cell genes from the active to inactive state during differentiation of embryonic stem cells.

  1. Sauvageau, M. and Sauvageau, G. (2010) Cell Stem Cell 7, 299-313.
  2. Brien, G.L. et al. (2012) Nat Struct Mol Biol 19, 1273-81.
  3. Ballaré, C. et al. (2012) Nat Struct Mol Biol 19, 1257-65.
  4. Cai, L. et al. (2013) Mol Cell 49, 571-82.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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