Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

CHD8 Antibody #7656

CHD   CHD-8   CHD8   chromatin helicase   Chromatin helicase DNA binding protein 8  

No. Size Price
7656S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
7656 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 290 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

CHD8 Antibody recognizes endogenous levels of total CHD8 protein.

CHD8 Antibody能够检测内源性的CHD8总蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human CHD8 protein. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from SH-SY5Y and HeLa cells using CHD8 Antibody.

使用CHD8 Antibody,免疫印迹(Western blot)分析SH-SY5Y和HeLa细胞中CHD8蛋白水平。


CHD8 belongs to the chromodomain helicase DNA-binding (CHD) family of ATP-dependent chromatin remodeling proteins (1). The CHD family of proteins has been shown to play an important role in regulating gene expression by utilizing the energy derived from ATP hydrolysis to alter chromatin architecture (1,2). The nine CHD family members are characterized by the presence of two tandem chromodomains in the N-terminal region and an SNF2-like ATPase domain near the central region of the protein (2-4). In addition, CHD8 contains three CR (conserved region) domains, a SANT (switching-defective protein 3, adaptor 2, nuclear receptor co-repressor, transcription factor IIB)-like domain, two BRK (brahma and kismet) domains and a DNA-binding domain (2). The chromatin remodeling activity of CHD8 has been shown to be important for the regulation of a wide variety of genes, such as the HOX genes (5) and genes that are driven by β-catenin (6), p53 (7), estrogen receptor (8), or androgen receptor (9). CHD8 can also interact with the insulator binding protein CTCF and is required for CTCF insulator activity at multiple gene loci (10).

CHD8属于ATP依赖的染色质重塑蛋白的chromodomain helicase DNA-binding (CHD)家族 (1)。研究证明CHD蛋白家族通过利用ATP水解的能量去改变染色质结构从而在调节基因表达中起到重要作用(1,2)。九种CHD家族成员都通过在N-端区域有两个串联的chromodomain和在蛋白的中间区域一个SNF2-like ATPase结构域存在为特征 (2-4)。因此,CHD8包含三个CR (conserved region)结构域、一个SANT (switching-defective protein 3, adaptor 2, nuclear receptor co-repressor, transcription factor IIB)-like结构域、两个BRK (brahma and kismet)结构域和一个DNA结合结构域 (2)。研究显示CHD8蛋白的染色质重塑活性对于很多基因调节是重要的,例如HOX基因(5),并且这些基因通过β-catenin (6)、p53 (7)、estrogen receptor (8)或androgen receptor (9)被调节。CHD8蛋白也能够与绝缘子结合蛋白质CTCF相互作用,并且在多种基因位点CTCF绝缘子活性是需要的(10)。

  1. Hargreaves, D.C. and Crabtree, G.R. (2011) Cell Res 21, 396-420.
  2. Marfella, C.G. and Imbalzano, A.N. (2007) Mutat Res 618, 30-40.
  3. Delmas, V. et al. (1993) Proc Natl Acad Sci U S A 90, 2414-8.
  4. Woodage, T. et al. (1997) Proc Natl Acad Sci U S A 94, 11472-7.
  5. Yates, J.A. et al. (2010) FEBS Lett 584, 689-93.
  6. Thompson, B.A. et al. (2008) Mol Cell Biol 28, 3894-904.
  7. Nishiyama, M. et al. (2009) Nat Cell Biol 11, 172-82.
  8. Caldon, C.E. et al. (2009) Mol Cell Biol 29, 4623-39.
  9. Menon, T. et al. (2010) Mol Endocrinol 24, 1165-74.
  10. Ishihara, K. et al. (2006) Mol Cell 23, 733-42.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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