Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb #7649


No. Size Price
7649L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
7649S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
7649T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
7649 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 84, 91 Rabbit IgG
IP 1:50
F 1:200
IF-IC 1:50
ChIP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP,


Species predicted to react based on 100% sequence homology: Monkey,

Specificity / Sensitivity

Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb recognizes endogenous levels of Stat1 protein only when phosphorylated at Tyr701.

Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb 只能检测内源的在Tyr701位点磷酸化的Stat1蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr701 of human Stat1 protein.

此单克隆抗体是通过合成人源对应的Stat1 Tyr701位点周围的肽段来免疫动物而获得。



Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with hIFN-α1 #8927 (100 ng/mL, 30 min; right), using Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red).共聚焦免疫荧光分析HeLa细胞,未经处理 (左图) 和经 hIFN-α1 #8927 (100 ng/mL, 30 min; 右图)处理,所用抗体为Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb (绿色)和β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (红色)。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with hIFN-α1 #8927 (green), using Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb.流式细胞仪分析Jurkat细胞未经处理 (蓝色)或经过hIFN-α1 #8927 处理(绿色),所用抗体为Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HT-1080 cells treated with IFN-γ (50 ng/ml) for 30 minutes and either 5 μl of Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP® Human TAP1 Promoter Primers #5148, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫共沉淀。HT-1080细胞培养至4 x 106, 并经IFN-γ (50 ng/ml) 处理30分钟,然后用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003进行免疫沉淀实验,本实验中用5 μl Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb 或2 μl Normal Rabbit IgG #2729 。用人的IRF-1启动子引物和SimpleChIP® Human TAP1 Promoter Primers #5148,SimpleChIP® Human α Satellite Repeat Primers #4486对富集的DNA做real-time PCR。每个样本中沉淀的DNA量定义为相对信号与输入的总染色质相比的数值。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, A20, and PC-12 cells, untreated or treated with Human Interferon-α1 (hIFN-α1) #8927 (10 ng/ml, 30 min), using Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb (upper) or Stat1 Antibody #9172 (lower).Western免疫印迹。用Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb (上图) 或者 Stat1 Antibody #9172 (下图)研究未经处理的和经hIFN-α1 #8927 (10 ng/ml, 30 min)处理的 HeLa, A20和PC-12 细胞的细胞提取液。


The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.

Stat1转录因子可以被一系列配体所激活(1), 其中对IFN-α和 IFN-γ的应答非常重要(2,3)。 在 Tyr701位点的磷酸化诱导 Stat1形成二聚体, 入核后与DNA结合(4)。 Stat1 蛋白是以Stat1α (91 kDa) 和 剪接变体Stat1β (84 kDa)二聚体的形式存在。在很多细胞中,这两种形式都是通过IFN-α激活。但是只有 Stat1α是被 IFN-γ激活。Stat1这种不正常的激活方式存在于很多肿瘤(5)。除了酪氨酸磷酸化,Stat1 也能通过p38有丝分裂激活蛋白激酶依赖(MAPK)依赖的通路在Ser727位点发生磷酸化而被激活,从而对IFN-α和其它的细胞应激产生应答(6)。丝氨酸磷酸化对最大化的诱导Stat1介导的基因激活也是必需的。

  1. Heim, M.H. (1999) J Recept Signal Transduct Res 19, 75-120.
  2. Durbin, J.E. et al. (1996) Cell 84, 443-50.
  3. Meraz, M.A. et al. (1996) Cell 84, 431-42.
  4. Ihle, J.N. et al. (1994) Trends Biochem Sci 19, 222-7.
  5. Frank, D.A. (1999) Mol Med 5, 432-56.
  6. Wen, Z. et al. (1995) Cell 82, 241-50.

Application References

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