Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

HDAC6 (D21B10) Rabbit mAb #7612

No. Size Price
7612S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
7612 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 160 Rabbit IgG
IP 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

HDAC6 (D21B10) Rabbit mAb recognizes endogenous levels of total HDAC6 protein.

HDAC6 (D21B10) Rabbit mAb兔单抗能够检测内源性HDAC6总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro681 of human HDAC6 protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from C2C12 cells, wild-type mouse embryonic fibroblasts (MEFs), and HDAC6 (-/-) MEFs using HDAC6 (D21B10) Rabbit mAb (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower).

使用HDAC6 (D21B10) Rabbit mAb 兔单抗(上图) or β-Actin (13E5) Rabbit mAb #4970 兔单抗(下图),免疫印迹(Western blot)分析C2C12和H-4-II-E细胞中HDAC6 (D21B10)的蛋白水平。

Western Blotting

Western Blotting

Western blot analysis of extracts from C2C12 and H-4-II-E cells using HDAC6 (D21B10) Rabbit mAb.

使用HDAC6 (D21B10) Rabbit mAb兔单抗,免疫印迹(Western blot)分析C2C12和H-4-II-E细胞中HDAC6 (D21B10)的蛋白水平。


HDAC6 is a class II histone deacetylase enzyme localized to the cytoplasm and associated with the microtubule network (1). It is involved in the regulation of many cellular processes, including cell migration, immune synapse formation, viral infection, and degradation of misfolded proteins (1). HDAC6 contains two tandem catalytic domains that facilitate the deacetylation of multiple protein substrates, including histones and non-histone proteins such as tubulin, cortactin, and HSP90. Despite the ability to deacetylate histone proteins in vitro, there is no evidence for HDAC6-mediated deacetylation of histones in vivo (2,3). The acetylation/deacetylation of tubulin on Lys40 regulates binding and motility of the kinesin-1 motor protein and subsequent transport of cargo proteins such as JNK-interacting protein 1 (JIP1) (4). The acetylation/deacetylation of cortactin regulates cell motility by modulating the binding of cortactin to F-actin (5). Acetylation/deacetylation of HSP90 modulates chaperone complex activity by regulating the binding of an essential cochaperone protein, p23 (6,7). In addition to its role as a protein deacetylase, HDAC6 functions as a component of the aggresome, a proteinaceous inclusion body that forms in response to an accumulation of misfolded or partially denatured proteins (8). Formation of the aggresome is a protective response that sequesters cytotoxic protein aggregates for eventual autophagic clearance from the cell. HDAC6 contains a zinc finger ubiquitin-binding domain that binds both mono- and poly-ubiquitinated proteins (8). HDAC6 binds to both poly-ubiquitinated misfolded proteins and dynein motors, facilitating the transport of misfolded proteins to the aggresome (9,10). HDAC6 is also required for subsequent recruitment of the autophagic machinery and clearance of aggresomes from the cell (11). Thus, HDAC6 plays a key role in the protection against the deleterious effects of pathological protein aggregation that occurs in various diseases, such as neurodegenerative Huntington’s disease (11).

HDAC6蛋白是一个class II组蛋白去乙酰化酶,它定位于细胞质和相关的微管网络(1)。它涉及调节许多细胞内进程,包括细胞迁移、免疫突触的形成、病毒性感染和错误折叠蛋白的降解 (1)。HDAC6包含两个串联的催化区域,这些结构域能有助于多种蛋白底物的去乙酰化,包括组蛋白和非组蛋白例如tubulin、cortactin和HSP90。尽管在体外具有组蛋白去乙酰化的能力,但是没有证据证明在体内HDAC6可介导组蛋白去乙酰化(2,3)。tubulin蛋白在Lys40位点乙酰化/去乙酰化可调节kinesin-1动力蛋白的结合和能动性,以及随后货运蛋白的转运例如JNK-interacting protein 1 (JIP1) (4)。通过介导cortactin蛋白到F-actin的结合,cortactin蛋白的乙酰化/去乙酰化可调节细胞能动性(5)。通过调节一个重要共同伴侣蛋白p23的结合,HSP90蛋白的乙酰化/去乙酰化可调节伴侣复合物活性(6,7)。除了HDAC6作为蛋白质去乙酰化酶之外,HDAC6蛋白还作为聚集小体的一个成分,它是一个蛋白质包含体,其在错误折叠或部分变性蛋白的堆积反应中形成(8)。聚集小体的形成是一个保护性反应,它对于细胞最终的自噬清除隔绝细胞毒性蛋白的聚集。HDAC6蛋白包含一个锌指机构泛素结合域,该结构域恩呢该结合单和多泛素化蛋白(8)。HDAC6蛋白结合到多泛素化错误折叠蛋白和动力蛋白,这有助于错误折叠蛋白转运到聚集小体(9,10)。HDAC6蛋白也对于自噬系统的招募和细胞聚集小体的清除都是需要的(11)。因此,HDAC6蛋白在多种疾病中的致病蛋白聚集的有害影响的保护起着重要作用,例如神经退行性疾病亨廷顿病(11)。

  1. Boyault, C. et al. (2007) Oncogene 26, 5468-76.
  2. Haggarty, S.J. et al. (2003) Proc Natl Acad Sci U S A 100, 4389-94.
  3. Zhang, Y. et al. (2003) EMBO J 22, 1168-79.
  4. Reed, N.A. et al. (2006) Curr Biol 16, 2166-72.
  5. Zhang, X. et al. (2007) Mol Cell 27, 197-213.
  6. Kovacs, J.J. et al. (2005) Mol Cell 18, 601-7.
  7. Murphy, P.J. et al. (2005) J Biol Chem 280, 33792-9.
  8. Seigneurin-Berny, D. et al. (2001) Mol Cell Biol 21, 8035-44.
  9. Kawaguchi, Y. et al. (2003) Cell 115, 727-38.
  10. Boyault, C. et al. (2006) EMBO J 25, 3357-66.
  11. Iwata, A. et al. (2005) J Biol Chem 280, 40282-92.

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