Cell Signaling Technology

Product Pathways - Neuroscience

mGluR2 (D7D8M) Rabbit mAb #76012

GPCR   metabotropic  

No. Size Price
76012S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
76012 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 100, >200 Rabbit IgG
IP 1:50
IF-F 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-F=Immunofluorescence (Frozen),

Specificity / Sensitivity

mGluR2 (D7D8M) Rabbit mAb recognizes endogenous levels of total mGluR2 protein. This antibody may cross-react with mGluR3.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular loops of mouse mGluR2 protein.

IF-F

IF-F

Confocal immunofluorescent analysis of rat hippocampus (left) and cerebellum (right) using mGluR2 (D7D8M) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IP

IP

Immunoprecipitation of mGluR2 from mouse brain extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is mGluR2 (D7D8M) Rabbit mAb. Western blot analysis was performed using mGluR2 (D7D8M) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various tissues using mGluR2 (D7D8M) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Background

Metabotropic glutamate receptor 2 (mGluR2) is a class C G protein-coupled receptor for the neurotransmitter glutamate in the mammalian brain. Unlike ionotropic receptors, metabotropic receptors do not form an ion channel pore themselves but are indirectly linked to ion channels (1). While mGluR1 and mGluR5 activate phospholipase C, mGluR2, mGluR3, mGluR4, and mGluR6 are coupled to the inhibitory G protein Gα(i/o) and inhibit adenylyl cyclase (AC) activity (1). Research studies have suggested that mGluR2/3 receptors may be potential targets for the treatment of Schizophrenia (2). Furthermore, mGluR2 interacts with the 5HT2A serotonin receptor to form a hetero-complex in the brain. This complex is a potential pharmacological target for hallucinogenic drugs (3,4).

  1. Pin, J.P. et al. (1994) EMBO J 13, 342-8.
  2. Patil, S.T. et al. (2007) Nat Med 13, 1102-7.
  3. González-Maeso, J. et al. (2008) Nature 452, 93-7.
  4. González-Maeso, J. and Sealfon, S.C. (2009) Trends Neurosci 32, 225-32.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

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