Cell Signaling Technology

Product Pathways - Adhesion

γ-Catenin (D9M1Q) Rabbit mAb #75550

No. Size Price
75550S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
75550 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 83 Rabbit IgG
IP 1:50
IHC-P 1:1000
F 1:100
IF-IC 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

γ-Catenin (D9M1Q) Rabbit mAb recognizes endogenous levels of total γ-Catenin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro725 of human γ-Catenin protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of DLD-1 (positive; left) and U-87 MG (negative; right) cells using γ-Catenin (D9M1Q) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of U-87 MG cells (blue) and DLD-1 cells (green) using γ-Catenin (D9M1Q) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded DLD-1 and U-87 MG cell pellets using γ-Catenin (D9M1Q) Rabbit mAb #75550.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using γ-Catenin (D9M1Q) Rabbit mAb (upper) or α-Actinin (D6F6) Rabbit mAb #6487 (lower).

IP

IP

Immunoprecipitation of γ-Catenin from DLD-1 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is γ-Catenin (D9M1Q) Rabbit mAb. Western blot was performed using γ-Catenin (D9M1Q) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using γ-Catenin (D9M1Q) Rabbit mAb #75550.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using γ-Catenin (D9M1Q) Rabbit mAb #75550.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using γ-Catenin (D9M1Q) Rabbit mAb #75550.

Background

Also known as plakoglobin, γ-catenin is a member of the Armadillo family of signaling molecules, which includes β-catenin and the Drosophila protein armadillo (1). This family of proteins is involved in Wnt signaling, which is important in embryonic development and in tumorigenesis (2-3). Although the two vertebrate proteins β- and γ-catenin display sequence homology, γ-catenin likely plays a role distinct from that of β-catenin (1, 4-6). γ-catenin localizes to desmosomes and adherens junctions, both sites of intercellular adhesion, and interacts with the cytoplasmic domains of classical and desmosomal cadherins. Interaction of γ- or β-catenin with α-catenin, desmoplakin and other junction proteins provides a link between intercellular junctions and the actin and intermediate filament cytoskeleton. Maintenance and/or modification of this link is vital for control of cell adhesion and migration (1). γ-catenin is modified by phosphorylation, affecting both adhesion and β-catenin dependent transcription (7), and by and O-glycosylation, affecting adhesion (8). Recent evidence suggests that γ-catenin regulates desmosomal adhesion in response to growth factor stimulation (9).

  1. Zhurinsky, J. et al. (2000) J Cell Sci 113 ( Pt 18), 3127-39.
  2. Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
  3. Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
  4. Zhurinsky, J. et al. (2000) Mol Cell Biol 20, 4238-52.
  5. Charpentier, E. et al. (2000) J Cell Biol 149, 503-20.
  6. Kolligs, F.T. et al. (2000) Genes Dev 14, 1319-31.
  7. Miravet, S. et al. (2003) Mol Cell Biol 23, 7391-402.
  8. Hu, P. et al. (2006) J Biol Chem 281, 12786-91.
  9. Yin, T. et al. (2005) J Biol Chem 280, 40355-63.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

SignalStain is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications