Cell Signaling Technology

Product Pathways - Stem Cell and Lineage Markers

Oct-4 (D7O5Z) Mouse mAb #75463

sc-25401   sc-5279   sc-8628   sc-9081  

No. Size Price
75463S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
75463 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 45 Mouse IgG1
F 1:50
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Oct-4 (D7O5Z) Mouse mAb recognizes endogenous levels of total Oct-4 protein. This antibody does not perform optimally on mouse cells in immunofluorescence assays.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe38 of human Oct-4 protein.



Confocal immunofluorescent analysis of NTERA-2 (left) and HeLa (right) cells using Oct-4 (D7O5Z) Mouse mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). As expected, HeLa cells are negative for Oct-4 expression.

Western Blotting

Western Blotting

Western blot analysis of extracts from NTERA-2 and HeLa cells using Oct-4 (D7O5Z) Mouse mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). As expected, HeLa cells are negative for Oct-4 expression.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells (blue) and NTERA cells (green) using Oct-4 (D7O5Z) Mouse mAb. Anti-mouse IgG (H+L), F(ab')2 fragment (Alexa Fluor 488 conjugate) #4408 was used as a secondary Ab.


Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells (1). A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Research studies have demonstrated that Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that the Oct-4A isoform has the ability to confer and sustain pluripotency, while Oct-4B may exist in some somatic, non-pluripotent cells (6,7).

  1. Looijenga, L.H. et al. (2003) Cancer Res 63, 2244-50.
  2. Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-278.
  3. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9.
  4. Cheng, L. et al. (2007) J Pathol 211, 1-9.
  5. Takeda, J. et al. (1992) Nucleic Acids Res 20, 4613-20.
  6. Cauffman, G. et al. (2006) Stem Cells 24, 2685-91.
  7. Lee, J. et al. (2006) J Biol Chem 281, 33554-65.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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