Cell Signaling Technology

Product Pathways - Development

Pontin/RUVBL1 (D1L8J) XP® Rabbit mAb #74775

No. Size Price
74775S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
74775T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
74775 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 50 Rabbit IgG
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, ChIP=Chromatin IP,

Specificity / Sensitivity

Pontin/RUVBL1 (D1L8J) XP® Rabbit mAb recognizes endogenous levels of total Pontin/RUVBL1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu428 of human Pontin/RUVBL1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Pontin/RUVBL1 (D1L8J) XP® Rabbit mAb.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HCT-15 cells treated with Human Interferon-α1 (hIFN-α1) #8927 (100 ng/ml; 45 min) and either 10 μl of Pontin/RUVBL1 (D1L8J) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human C/EBP-δ Primers #48781 and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Background

Reptin/RuvBL2 and Pontin/RuvBL1 are closely related members of the AAA+ (ATPase associated with diverse cellular activities) superfamily of proteins, and are putatively homologous to bacterial RuvB proteins that drive branch migration of Holliday junctions (1). Reptin and Pontin function together as essential components of chromatin remodeling and modification complexes, such as INO80, TIP60, SRCAP, and Uri1, which play key roles in regulating gene transcription (1,2). In their capacity as essential transcriptional co-regulators, Reptin and Pontin have both been implicated in oncogenic transformations, including those driven by c-Myc, β-catenin, and E1A (2-7).

  1. Jha, S. and Dutta, A. (2009) Mol Cell 34, 521-33.
  2. Gallant, P. (2007) Trends Cell Biol 17, 187-92.
  3. Huber, O. et al. (2008) Cancer Res 68, 6873-6.
  4. Kim, J.H. et al. (2005) Nature 434, 921-6.
  5. Bauer, A. et al. (2000) EMBO J 19, 6121-30.
  6. Wood, M.A. et al. (2000) Mol Cell 5, 321-30.
  7. Dugan, K.A. et al. (2002) Oncogene 21, 5835-43.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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