Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

TBL1XR1/TBLR1 (D4J9C) Rabbit mAb #74499


No. Size Price
74499S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
74499 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 50, 60 Rabbit IgG
IP 1:100
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP,

Specificity / Sensitivity

TBL1XR1/TBLR1 (D4J9C) Rabbit mAb recognizes endogenous levels of total TBL1XR1/TBLR1 protein. This antibody also cross-reacts with an unidentified protein of 130 kDa.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro159 of human TBL1XR1/TBLR1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-29, RPMI 8226 and COS-7 cells using TBL1XR1/TBLR1 (D4J9C) Rabbit mAb.



Immunoprecipitation of TBL1XR1/TBLR1 from HT-29 cell extracts. Lane 1 is 10% input. Lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 and lane 3 is TBL1XR1/TBLR1 (D4J9C) Rabbit mAb. Western blot analysis was performed using TBL1XR1/TBLR1 (D4J9C) Rabbit mAb.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HCT 116 cells and either 10 μl of TBL1XR1/TBLR1 (D4J9C) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Axin2 Intron 1 Primers #8973, SimpleChIP® Human CaMK2D Intron 3 Primers #5111, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


Transducing β-like protein 1 (TBL1X/TBL1) and TBL1-related protein 1 (TBL1XR1/TBLR1) were originally identified as subunits of the co-repressor silencing mediator for retinoic and thyroid hormone receptor (SMRT) and nuclear receptor co-repressor (NCoR) complexes (1-3). These two factors are required for the exchange of co-repressor complexes for co-activators by acting as adaptors to recruit the ubiquitin/proteasome machinery that degrades the co-repressor proteins during ligand mediated activation of transcription (4,5). Co-factor exchange driven by TBL1X/TBL1 and TBL1XR1/TBLR1 appears to be the mechanism by which c-Jun and NF-κB mediated transcription is activated and is therefore likely to be the mechanism employed by other signal-dependent transcription factors as well (4,6). In addition, both TBL1X/TBL1 and TBL1XR1/TBLR1 have essential roles in regulating the Wnt-signaling pathway by recruiting β-catenin to Wnt target genes to activate transcription. Depletion of TBL1X-TBL1XR1 significantly inhibited Wnt-beta-catenin- induced gene expression and oncogenic growth in vitro and in vivo (7). Research studies have shown that upregulation of TBL1XR/TBLR1 is observed in a variety of solid tumors, and is correlated with advanced tumor stage, metastasis and poor prognosis (1).

  1. Li, J.Y. et al. (2015) Am J Clin Exp Urol 3, 13-23.
  2. Zhang, J. et al. (2002) Mol Cell 9, 611-23.
  3. Yoon, H.G. et al. (2003) EMBO J 22, 1336-46.
  4. Perissi, V. et al. (2004) Cell 116, 511-26.
  5. Perissi, V. et al. (2008) Mol Cell 29, 755-66.
  6. Hoberg, J.E. et al. (2004) Mol Cell 16, 245-55.
  7. Li, J. and Wang, C.Y. (2008) Nat Cell Biol 10, 160-9.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :