Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

CBP (D9B6) Rabbit mAb #7425

chip-seq   sc-369  

No. Size Price
7425S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
7425 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 300 Rabbit IgG
IP 1:200
ChIP 1:50
ChIP-seq 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP, ChIP-seq=Chromatin IP-seq,

Specificity / Sensitivity

CBP (D9B6) Rabbit mAb recognizes endogenous levels of total CBP protein. This antibody also shows some cross-reactivity with p300 protein.

CBP (D9B6) Rabbit mAb兔单抗能够检测内源性CBP总蛋白水平。该抗体也显示与p300蛋白发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human CBP protein.


Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 293 cells, treated with Forskolin #3828 (30 μM) for 1h, and either 10 μl of CBP (D9B6) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP® Human NR4A3 Promoter Primers #4829, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

使用SimpleChIP®Enzymatic Chromatin IP Kit (Magnetic Beads) #9003,来自Forskolin #3828 (30uM)处理过的4 x 106 293细胞的交联染色质以及10 µl CBP (D9B6) Rabbit mAb或2 µl Normal Rabbit IgG #2729进行染色质免疫沉淀实验。使用human ALS2 exon 1 primers、SimpleChIP® Human NR4A3 Promoter Primers #4829和SimpleChIP® Human α Satellite Repeat Primers #4486,浓缩的DNA通过real-time PCR定量。在每个样品中免疫沉淀DNA的数量被当做一个相对于总input chromatin的数量的信号,这相当于一。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CBP (D9B6) Rabbit mAb.

使用CBP (D9B6) Rabbit mAb兔单抗,免疫印迹(Western blot)分析不同细胞的CBP蛋白水平。

Chromatin IP-seq

Chromatin IP-seq

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HCT116 cells and 10 μl of CBP (D9B6) Rabbit mAb, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. DNA Libraries were prepared from 5ng enriched ChIP DNA using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®, and sequenced on the Illumina NextSeq. The figure shows binding across ALS2, a known target gene of CBP (see additional figure containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product data sheet.


CBP (CREB-binding protein) and p300 are highly conserved and functionally related transcriptional co-activators that associate with transcriptional regulators and signaling molecules, integrating multiple signal transduction pathways with the transcriptional machinery (1,2). CBP/p300 also contain histone acetyltransferase (HAT) activity, allowing them to acetylate histones and other proteins (2). Phosphorylation of p300 at Ser89 by PKC represses its transciptional acitivity, and phosphorylation at the same site by AMPK disrupts the association of p300 with nuclear receptors (3,4). Ser1834 phosphorylation of p300 by Akt disrupts its association with C/EBPβ (5). Growth factors induce phosphorylation of CBP at Ser437, which is required for CBP recruitment to the transcription complex (6). CaM kinase IV phosphorylates CBP at Ser302, which is required for CBP-dependent transcriptional activation in the CNS (7). The role of acetylation of CBP/p300 is of particular interest (2,8). Acetylation of p300 at Lys1499 has been demonstrated to enhance its HAT activity and affect a wide variety of signaling events (9).

CBP (CREB-binding protein)和p300蛋白是高度保守的并且具有与转录共激活因子有关联的功能,它是与转录调节因子和信号分子有关联,使用转录分子去整合多种信号转导通路(1,2)。CBP/p300也包含组蛋白乙酰转移酶(HAT)活性,它允许它们使组蛋白或其它蛋白发生乙酰化(2)。通过PKC使p300蛋白在Ser89位点的磷酸化抑制它的转录活性,并且通过AMPK使相同位点的磷酸化干扰了细胞核受体与p300蛋白的联系(3,4)。Akt使p300蛋白Ser1834位点的磷酸化干扰它与C/EBPβ的联系(5)。生长因子可诱导CBP在Ser437位点磷酸化,这对于CBP被招募到转录复合物上时需要的(6)。CaM激酶IV 使CBP蛋白的Ser302位点磷酸化,这在中枢神经系统中对于CBP依赖的转录活性是需要的(7)。CBP/p300蛋白乙酰化的作用是特别有兴趣(2,8)。p300蛋白Lys1499位点的乙酰化已经被证明能提高它的HAT活性,并且影响很多信号传导(9)。

  1. Goodman, R.H. and Smolik, S. (2000) Genes Dev 14, 1553-77.
  2. Chan, H.M. and La Thangue, N.B. (2001) J. Cell Sci. 114, 2363-2373.
  3. Yuan, L.W. and Gambee, J.E. (2000) J. Biol. Chem. 275, 40946-40951.
  4. Yang, W. et al. (2001) J. Biol. Chem. 276, 38341-38344.
  5. Guo, S. et al. (2001) J. Biol. Chem. 276, 8516-8523.
  6. Zanger, K. et al. (2001) Mol. Cell 7, 551-558.
  7. Impey, S. et al. (2002) Neuron 34, 235-244.
  8. Yuan, L.W. and Giordano, A. (2002) Oncogene 21, 2253-2260.
  9. Thompson, P.R. et al. (2004) Nat. Struct. Mol. Biol. 11, 308-315.

Application References

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