Cell Signaling Technology

Product Pathways - Nuclear Receptor Signaling

Androgen Receptor (D6F11) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #7395

No. Size Price
7395S 100 µl ( 50 tests ) ¥4,264.00 现货查询 购买询价
7395 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human, Endogenous Rabbit IgG
IF-IC 1:50

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Androgen Receptor (D6F11) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total androgen receptor protein.Androgen Receptor (D6F11) XP® Rabbit mAb (Alexa Fluor® 488结合)能够检测内源性水平的总人雄激素受体蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein corresponding to residues near the amino terminal region of human androgen receptor protein.该单克隆抗体是由对应人雄激素受体蛋白靠近氨基末端残基的重组蛋白免疫动物而生产的。

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Androgen Receptor (D6F11) XP® Rabbit mAb #5153.该Cell Signaling Technology抗体与Alexa Fluor® 488荧光染料结合,并直接用流式细胞术内部测试检测人体细胞。预计该抗体与非结合的Androgen Receptor (D6F11) XP® Rabbit mAb #5153一样,表现出相同的物种交叉反应。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat (blue) and LNCaP (green) cells using Androgen Receptor (D6F11) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate).流式细胞术检测Jurkat细胞 (蓝色)和LNCaP细胞 (绿色),使用的抗体为 Androgen Receptor (D6F11) XP® Rabbit mAb (Alexa Fluor® 488 结合).

IF-IC

IF-IC

Confocal immunofluorescent analysis of LNCaP (left) and DU 145 (right) cells using Androgen Receptor (D6F11) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) (green) and Hexokinase I (C35C4) Rabbit mAb (Alexa Fluor® 647 Conjugate) #3540 (blue pseudocolor). Actin filaments were labeled with DY-554 phalloidin (red).激光共聚焦免疫荧光方法检测 LNCaP 细胞(左图) 和DU 145 细胞(右图),使用的抗体为Androgen Receptor (D6F11) XP® Rabbit mAb (Alexa Fluor® 488 结合) (绿色) 和Hexokinase I (C35C4) Rabbit mAb (Alexa Fluor® 647 结合) #3540 (蓝色伪彩色). 肌动蛋白丝被DY-554鬼笔环肽标记(红色)。

Background

Androgen receptor (AR), a zinc finger transcription factor belonging to the nuclear receptor superfamily, is activated by phosphorylation and dimerization upon ligand binding (1). This promotes nuclear localization and binding of AR to androgen response elements in androgen target genes. AR plays a crucial role in several stages of male development and the progression of prostate cancer (2,3).雄激素受体(AR)是锌指转录因子,属于核受体超家族,通过磷酸化和配体结合的二聚化被激活(1)。这促进了细胞核定位和雄激素靶基因中雄激素与应答元件的结合。 AR在男性发育和前列腺癌发展的几个阶段起着至关重要的作用(2,3)。

  1. Li, J. and Al-Azzawi, F. (2009) Maturitas 63, 142-8.
  2. Avila, D.M. et al. (2001) J. Steroid. Biochem. Mol. Biol. 76, 135-142.
  3. Montgomery, J.S. et al. (2001) J. Pathol. 195, 138-146.

Application References

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Protocols


For Research Use Only. Not For Use In Diagnostic Procedures.

The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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