Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-Vimentin (Ser56) (D5H2) Rabbit mAb #7391

No. Size Price
7391S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
7391 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 57 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-Vimentin (Ser56) (D5H2) Rabbit mAb recognizes endogenous levels of vimentin protein only when phosphorylated at Ser56.

Phospho-Vimentin (Ser56) (D5H2)Rabbit mAb可以识别56位丝氨酸磷酸化的内源性vimentin蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser56 of human vimentin protein.

单克隆抗体由合成肽段免疫动物产生,该肽段与人vimentin蛋白56位丝氨酸附近氨基酸一致。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and C2C12 cells, hydroxyurea-treated (4 mM, G1/S arrested) or treated with Paclitaxel #9807 (100 nM, G2/M arrested) for 20 hr, using Phospho-Vimentin (Ser56) (D5H2) Rabbit mAb (upper) and Vimentin (D21H3) XP® Rabbit mAb #5741 (lower).经过Hydroxyurea处理(4 mM, G1/S arrested)或Paclitaxel #9807 (100 nM, G2/M arrested)处理20小时候的HeLa细胞和C2C12细胞提取物,使用Phospho-Vimentin (Ser56) (D5H2)Rabbit mAb (上) 和 Vimentin (D21H3) XP® Rabbit mAb #5741 (下)进行western blot分析。

Background

The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Major types of intermediate filaments are distinguished by their cell-specific expression: cytokeratins (epithelial cells), glial fibrillary acidic protein (GFAP) (glial cells), desmin (skeletal, visceral, and certain vascular smooth muscle cells), vimentin (mesenchyme origin), and neurofilaments (neurons). GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape (1). In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes (2). Research studies have shown that vimentin is present in sarcomas, but not carcinomas, and its expression is examined in conjunction with that of other markers to distinguish between the two (3). Vimentin's dynamic structural changes and spatial re-organization in response to extracellular stimuli help to coordinate various signaling pathways (4). Phosphorylation of vimentin at Ser56 in smooth muscle cells regulates the structural arrangement of vimentin filaments in response to serotonin (5,6). Remodeling of vimentin and other intermediate filaments is important during lymphocyte adhesion and migration through the endothelium (7).

细胞骨架由三种细胞纤维构成:微丝(肌动蛋白丝),中间丝和微管。中间丝的主要类型由它们的细胞特异性表达来区分:细胞角蛋白(上皮细胞),胶质原纤维酸性蛋白(GFAP)(神经胶质细胞),结蛋白(desmin)(骨骼,内脏,某些血管平滑肌细胞),波形蛋白(间充质起源),神经丝蛋白(神经元)。星形胶质细胞中GFAP和vimentin形成中间丝以调节它们的运动和形状(1)。尤其是vimentin中间丝出现在发育早期,GFAP中间丝是终末分化的成熟脑星形胶质细胞的标记(2)。研究表明vimentin在肉瘤中表达,而在肿瘤中不表达,它的表达与其他标记蛋白相结合用于研究区分这二者(3)。Vimentin的多变的结构和空间重组特性是对细胞外刺激的应激,帮助细胞协同调节多种信号通路(4)。肌肉细胞中Vimentin的56位丝氨酸磷酸化会调节vimentin中间丝的结构以应对肉瘤(5,6)。Vimentin和其他中间丝的重构对于淋巴细胞黏附及通过内皮细胞的迁移是非常重要的(7)。

  1. Eng, L.F. et al. (2000) Neurochem Res 25, 1439-51.
  2. Goebel, H.H. et al. (1987) Acta Histochem Suppl 34, 81-93.
  3. Leader, M. et al. (1987) Histopathology 11, 63-72.
  4. Helfand, B.T. et al. (2004) J Cell Sci 117, 133-41.
  5. Tang, D.D. et al. (2005) Biochem J 388, 773-83.
  6. Fomina, I.G. et al. (1990) Klin Med (Mosk) 68, 125-7.
  7. Nieminen, M. et al. (2006) Nat Cell Biol 8, 156-62.
  8. Yamaguchi, T. et al. (2005) J Cell Biol 171, 431-6.
  9. Oguri, T. et al. (2006) Genes Cells 11, 531-40.
  10. Zhu, Q.S. et al. (2011) Oncogene 30, 457-70.
  11. Xue, G. and Hemmings, B.A. (2013) J Natl Cancer Inst 105, 393-404.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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