Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-Vimentin (Ser56) (D5H2) Rabbit mAb #7391

No. Size Price
7391S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
7391 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 57 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-Vimentin (Ser56) (D5H2) Rabbit mAb recognizes endogenous levels of vimentin protein only when phosphorylated at Ser56.

Phospho-Vimentin (Ser56) (D5H2)Rabbit mAb可以识别56位丝氨酸磷酸化的内源性vimentin蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser56 of human vimentin protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and C2C12 cells, hydroxyurea-treated (4 mM, G1/S arrested) or treated with Paclitaxel #9807 (100 nM, G2/M arrested) for 20 hr, using Phospho-Vimentin (Ser56) (D5H2) Rabbit mAb (upper) and Vimentin (D21H3) XP® Rabbit mAb #5741 (lower).经过Hydroxyurea处理(4 mM, G1/S arrested)或Paclitaxel #9807 (100 nM, G2/M arrested)处理20小时候的HeLa细胞和C2C12细胞提取物,使用Phospho-Vimentin (Ser56) (D5H2)Rabbit mAb (上) 和 Vimentin (D21H3) XP® Rabbit mAb #5741 (下)进行western blot分析。


The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Major types of intermediate filaments are distinguished by their cell-specific expression: cytokeratins (epithelial cells), glial fibrillary acidic protein (GFAP) (glial cells), desmin (skeletal, visceral, and certain vascular smooth muscle cells), vimentin (mesenchyme origin), and neurofilaments (neurons). GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape (1). In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes (2). Research studies have shown that vimentin is present in sarcomas, but not carcinomas, and its expression is examined in conjunction with that of other markers to distinguish between the two (3). Vimentin's dynamic structural changes and spatial re-organization in response to extracellular stimuli help to coordinate various signaling pathways (4). Phosphorylation of vimentin at Ser56 in smooth muscle cells regulates the structural arrangement of vimentin filaments in response to serotonin (5,6). Remodeling of vimentin and other intermediate filaments is important during lymphocyte adhesion and migration through the endothelium (7).


  1. Eng, L.F. et al. (2000) Neurochem Res 25, 1439-51.
  2. Goebel, H.H. et al. (1987) Acta Histochem Suppl 34, 81-93.
  3. Leader, M. et al. (1987) Histopathology 11, 63-72.
  4. Helfand, B.T. et al. (2004) J Cell Sci 117, 133-41.
  5. Tang, D.D. et al. (2005) Biochem J 388, 773-83.
  6. Fomina, I.G. et al. (1990) Klin Med (Mosk) 68, 125-7.
  7. Nieminen, M. et al. (2006) Nat Cell Biol 8, 156-62.
  8. Yamaguchi, T. et al. (2005) J Cell Biol 171, 431-6.
  9. Oguri, T. et al. (2006) Genes Cells 11, 531-40.
  10. Zhu, Q.S. et al. (2011) Oncogene 30, 457-70.
  11. Xue, G. and Hemmings, B.A. (2013) J Natl Cancer Inst 105, 393-404.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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