Cell Signaling Technology

Product Pathways - Vesicle Trafficking

VPS29 (D2H7G) Rabbit mAb #73540

No. Size Price
73540S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
73540 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 21 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

VPS29 (D2H7G) Rabbit mAb recognizes endogenous levels of total VPS29 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala121 of human VPS29 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, untransfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human VPS29 (hVPS29-Myc/DDK; +), and of extracts from various cell lines and tissues, using VPS29 (D2H7G) Rabbit mAb.

Background

Retromer is a heteropentameric protein complex that consists of two protein compounds, a sortin-nexin dimer and a trimeric VPS26-VPS29-VPS35 protein subcomplex. The retromer complex associates with endosomes at their cytosolic side to mediate retrograde transport of transmembrane proteins from endosomes to the trans-Golgi network (1-3). Vacuolar protein sorting-associated protein 29 (VPS29) is considered a cryptic metallophosphoesterase, as it contains a conserved metallophosphoesterase-fold that includes a phenylalanine in place of an essential histidine residue within the active site (4). While VPS29 is capable of binding metal ions, it does so with low affinity and exhibits no enzymatic activity. Instead, VPS29 serves as a scaffold protein that interacts with the carboxy-terminal region of VPS35 and is essential for association of the retromer with other endosomal transport proteins (5).

  1. Seaman, M.N. (2005) Trends Cell Biol 15, 68-75.
  2. Bonifacino, J.S. and Rojas, R. (2006) Nat Rev Mol Cell Biol 7, 568-79.
  3. Verges, M. (2007) Front Biosci 12, 3825-51.
  4. Hierro, A. et al. (2007) Nature 449, 1063-7.
  5. Swarbrick, J.D. et al. (2011) PLoS One 6, e20420.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications