Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb #7237

No. Size Price
7237S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
7237T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
7237 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 37 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Monkey,

Specificity / Sensitivity

Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb recognizes endogenous levels of caspase-9 protein only when cleaved at Asp330.

Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb 兔单抗只能够检测内源的在Asp330位点剪切的caspase-9蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp330 of human caspase-9 protein.

单克隆抗体是采用合成的人源caspase-9 Asp330位点周围残基相对应的肽段来免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and Jurkat cells, untreated or treated with Staurosporine #9953 (1 μM, 3 hr) or etoposide (25 μM, overnight), using Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb (upper) or total Caspase-9 Antibody (Human Specific) #9502 (lower).

Western免疫印迹。用Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb 兔单抗(上图), 或者total Caspase-9 Antibody (Human Specific) #9502 (下图)研究未经处理或经 Staurosporine #9953 (1 μM, 3 hr) 或者etoposide (25 μM, 过夜)处理的HeLa 和Jurkat 细胞的细胞提取液。


Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Additional cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10).

Caspase-9 (ICE-LAP6, Mch6)是半胱氨酸天冬氨酸蛋白水解酶(caspase)家族的重要成员(1,2)。凋亡信号刺激后,细胞色素C从线粒体释放,这和47 kDa procaspase-9/Apaf 1有关。Apaf-1介导的caspase-9 活化,包括蛋白水解过程导致的caspase-9在Asp315处裂解并产生p35亚单位。另外一种裂解发生在Asp330处,产生p37亚单位,可以放大凋亡反应(3-6)。Cleaved caspase-9 进一步促进其他 caspase 家族成员的作用,包括 caspase-3 and caspase-7,并启动凋亡级联反应,最终导致凋亡。

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Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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