Product Pathways - PI3K / Akt Signaling
GSK-3β (D5C5Z) XP® Rabbit mAb (PE Conjugate) #72115
|72115S||100 µl ( 50 tests )||￥4,264.00 现货查询||购买询价|
|72115||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: F=Flow Cytometry,
Specificity / Sensitivity
GSK-3β (D5C5Z) XP® Rabbit mAb (PE Conjugate) recognizes endogenous levels of total GSK-3β protein. This antibody does not cross-react with GSK-3α protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human GSK-3β protein.
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in rat cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated GSK-3β (D5C5Z) XP® Rabbit mAb #12456.
Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).
- Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9.
- Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41.
- Cross, D.A. et al. (1995) Nature 378, 785-9.
- Nusse, R. (1997) Cell 89, 321-3.
- Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.
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- 12456 GSK-3β (D5C5Z) XP® Rabbit mAb
- 8466 Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb (PE Conjugate)
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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