Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

PDE4B (D7R1Y) Rabbit mAb #72096

No. Size Price
72096S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
72096 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 90 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

PDE4B (D7R1Y) Rabbit mAb recognizes endogenous levels of total PDE4B protein. This antibody does not cross-react with PDE4A protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu61 of human PDE4B protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse brain, rat brain, and A-204 cells using PDE4B (D7R1Y) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human PDE4B protein (hPDE4B-Myc/DDK; +) or Myc/DDK-tagged full-length human PDE4A protein (hPDE4A-Myc/DDK; +), using PDE4B (D7R1Y) Rabbit mAb (upper) and Myc-Tag (71D10) Rabbit mAb #2278 (lower).

IP

IP

Immunoprecipitation of PDE4B from mouse brain extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is PDE4B (D7R1Y) Rabbit mAb. Western blot analysis was performed using PDE4B (D7R1Y) Rabbit mAb.

Background

Enzymes of the phosphodiesterase (PDE) superfamily catalyze the hydrolysis of 3',5'-cyclic nucleotides into the corresponding nucleotide 5'-monophosphates. The PDE superfamily includes 11 subfamilies (PDE1-PDE11) in mammals (1). These enzymes function as important positive and negative regulators of cellular response, including regulation of insulin secretion, heart function, erectile function, and inflammation (2-5). The cAMP-specific phosphodiesterase 4B (PDE4B, DPDE4) is important for the inflammatory response to lipopolysaccharide in monocytes (6). PDE4B plays an important role in the hydrolysis and inactivation of the ubiquitous second messenger cAMP that regulates lymphocyte cell growth and apoptosis (7). Research studies indicate that PDE4B is also involved in psychiatric disorders, including schizophrenia, autism, and depression (8-10).

  1. Bender, A.T. and Beavo, J.A. (2006) Pharmacol Rev 58, 488-520.
  2. Dyachok, O. et al. (2006) Nature 439, 349-52.
  3. Fischmeister, R. et al. (2006) Circ Res 99, 816-28.
  4. Rosen, R.C. and Kostis, J.B. (2003) Am J Cardiol 92, 9M-18M.
  5. Smith, S.J. et al. (2004) Mol Pharmacol 66, 1679-89.
  6. Jin, S.L. and Conti, M. (2002) Proc Natl Acad Sci U S A 99, 7628-33.
  7. Conti, M. et al. (2003) J Biol Chem 278, 5493-6.
  8. Fatemi, S.H. et al. (2008) Schizophr Res 101, 36-49.
  9. Braun, N.N. et al. (2007) Neuroreport 18, 1841-4.
  10. Numata, S. et al. (2009) Am J Med Genet B Neuropsychiatr Genet 150B, 527-34.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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