Cell Signaling Technology

Product Pathways - Neuroscience

VGLUT2 (D7D2H) Rabbit mAb #71555

No. Size Price
71555S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
71555 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 65-70 Rabbit IgG
IP 1:50
IF-F 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-F=Immunofluorescence (Frozen),

Specificity / Sensitivity

VGLUT2 (D7D2H) Rabbit mAb recognizes endogenous levels of total VGLUT2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human VGLUT2 protein.



Confocal immunofluorescent analysis of mouse brain using VGLUT2 (D7D2H) Rabbit mAb (green) and β3-Tubulin (TU-20) Mouse mAb #4466 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).



Immunoprecipitation of VGLUT2 from mouse brain extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is VGLUT2 (D7D2H) Rabbit mAb. Western blot analysis was performed using VGLUT2 (D7D2H) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse brain, mouse lung, rat brain, and human cortex using VGLUT2 (D7D2H) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).


Glutamatergic neurons release glutamate, the most common excitatory neurotransmitter. Their synaptic vesicles are filled with glutamate by vesicular glutamate transporters, VGLUTs (1). VGLUT1, also called solute carrier family 17 member 7 (SLC17A7), was first identified as an inorganic phosphate transporter (2). Despite the absence of homology with neurotransmitter transporters, VGLUT1 was later demonstrated to be a glutamate transporter (1) specific to glutamatergic neurons (3). Closely related to VGLUT1, VGLUT2 and VGLUT3 are also involved in glutamate uptake into synaptic vesicles, but define different neuronal subpopulations (4,5). VGLUT1 and VGLUT2 are the most abundant isoforms. VGLUT1 is expressed in the cortex, hippocampus, and cerebellar cortex, while VGLUT2 is mostly found in the thalamus (6,7). VGLUT3 is expressed in hair cells of the auditory system (8).

  1. Bellocchio, E.E. et al. (2000) Science 289, 957-60.
  2. Ni, B. et al. (1996) J Neurochem 66, 2227-38.
  3. Takamori, S. et al. (2000) Nature 407, 189-94.
  4. Fremeau, R.T. et al. (2001) Neuron 31, 247-60.
  5. Fremeau, R.T. et al. (2002) Proc Natl Acad Sci U S A 99, 14488-93.
  6. Herzog, E. et al. (2001) J Neurosci 21, RC181.
  7. Kaneko, T. and Fujiyama, F. (2002) Neurosci Res 42, 243-50.
  8. Seal, R.P. et al. (2008) Neuron 57, 263-75.

Application References

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