Cell Signaling Technology

Product Pathways - Autophagy Signaling

Rubicon (D8B2) Rabbit mAb #7151

No. Size Price
7151S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
7151 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 130 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Rubicon (D8B2) Rabbit mAb recognizes endogenous levels of total Rubicon protein. Background bands of unknown origin are detected between 60 and 65 kDa.

Rubicon (D8B2) Rabbit mAb 兔单抗能识别内源性的Rubicon总蛋白。在60kDa和65kDa处检测到两条来源不明的条带。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Rubicon protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Rubicon (D8B2) Rabbit mAb.

Western blot 分析不同细胞系的提取物,使用的抗体是Rubicon (D8B2) Rabbit mAb 兔单抗。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with human Rubicon (hRubicon, +), using Rubicon (D8B2) Rabbit mAb. Rubicon construct was kindly provided by Dr. Qing Zhong, University of California, Berkeley, CA.

Western blot 分析293T细胞的提取物,对照转染(-)或人Rubicon (hRubicon, +)转染。使用的抗体是Rubicon (D8B2) Rabbit mAb 兔单抗。Rubicon 质粒由Dr. Qing Zhong, University of California, Berkeley, CA友情提供。


Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but is also associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and is directed by a number of autophagy-related (Atg) genes. These proteins are involved in the formation of autophagosomes, which are cytoplasmic vacuoles that are delivered to lysosomes for degradation. The class III type phosphoinositide 3-kinase (PI3K) Vps34 regulates vacuolar trafficking and autophagy (4,5). Multiple proteins associate with Vsp34, including p105/Vsp15, Beclin-1, UVRAG, Atg14, and Rubicon (6-12). Atg14L and Rubicon were identified based on their ability to bind to Beclin-1 and participate in unique complexes with opposing functions (9-12). Rubicon, which localizes to the endosome and lysosome, inhibits Vps34 lipid kinase activity; knockdown of Rubicon enhances autophagy and endocytic trafficking (11,12). In contrast, Atg14L localizes to autophagosomes, isolation membranes, and ER and can enhance Vps34 activity. Knockdown of Atg14L inhibits starvation-induced autophagy (11,12).


  1. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.
  2. Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18.
  3. Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679-88.
  4. Corvera, S. (2001) Traffic 2, 859-66.
  5. Yan, Y. and Backer, J.M. (2007) Biochem Soc Trans 35, 239-41.
  6. Stack, J.H. et al. (1995) J Cell Biol 129, 321-34.
  7. Zeng, X. et al. (2006) J Cell Sci 119, 259-70.
  8. Liang, C. et al. (2006) Nat Cell Biol 8, 688-99.
  9. Itakura, E. et al. (2008) Mol Biol Cell 19, 5360-72.
  10. Sun, Q. et al. (2008) Proc Natl Acad Sci USA 105, 19211-6.
  11. Zhong, Y. et al. (2009) Nat Cell Biol 11, 468-76.
  12. Matsunaga, K. et al. (2009) Nat Cell Biol 11, 385-96.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :