Cell Signaling Technology

Product Pathways - Companion Products

DTT (Dithiothreitol) #7016

agent   biology   buffer   disulfide   Dithiothreitol   molecular   reduce   reducing   reductant   sulfhydryl  

Description

Dithiothreitol (DTT) from Cell Signaling Technology is offered in a convenient 192.8 mg lyophilized format, allowing for preparation of a fresh stock solution. This DTT reagent contains no detectable DNase or RNase activity and is suitable for use in molecular biology or protein biochemistry applications that require reduction of protein disulfide bonds.

SDS-PAGE sample buffers are routinely supplemented with 10-50 mM DTT to cleave protein disulfide bonds. Lower concentrations of DTT are routinely used to stabilize enzymes or other proteins that posses free sulfhydryl groups, which is useful in chromatin immunoprecipitation (ChIP) assays.

Molecular Characterization

C4H10O2S2

Purity

>99%

Structure

Structure

Chemical structure of DTT.

Directions for Use

DTT is supplied as a lyophilized powder. To prepare a 1 M stock solution, reconstitute the 192.8 mg supplied in the product tube in 1.12 ml ddH2O. The dissolved DTT will increase the final volume to approximately 1.25 ml.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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