Cell Signaling Technology

Product Pathways - PathScan ELISA

STOP Solution #7002

ELISA  

No. Size Price
7002L 500 ml ( 50 x 96 well plates ) ¥1,284.00 现货查询 购买询价
7002S 100 ml ( 10 x 96 well plates ) ¥370.00 现货查询 购买询价
7002 carrier free & custom formulation / quantityemail request
Applications Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Description

STOP Solution is a proprietary solution used to terminate the peroxidase/TMB reaction for ELISA applications. The TMB substrate reacts with immobilized horseradish peroxidase (HRP) conjugated secondary antibodies to produce a blue solution. Color intensity is an indication of analyte level. After attaining the desired intensity, the reaction is terminated by addition of STOP Solution. Upon addition of STOP Solution the color turns from blue to yellow. Absorbance at 450 nm can be read immediately, and he reaction is stable for one hour.

终止液在ELISA中用于终止过氧化物酶/TMB的反应。底物和固定的辣根过氧化物酶(HRP)结合的二抗之间的反应生成蓝色的溶液。颜色的深度与分析物的含量成比例。达到所需要的颜色深度后,加入酸性终止液可以使反应溶液的颜色右蓝色变为黄色。终止反应1小时内,结果可以保持稳定。板子应该在尽快用读板机于450nm读取并分析结果。

Directions for Use

(a) After incubation with horseradish peroxidase (HRP) conjugated secondary antibody, wash plates with PBS/0.05% Tween-20. 
 (b) Add 100 μl of TMB solution/well. Incubate for a specific amount of time or until desired signal is achieved. See product datasheet for recommended incubation times for specific antibody pair. 
 (c) Add 100 μl of STOP Solution. 
 (d) Read at Absorbance 450 nm.

(a)与辣根过氧化物酶(HRP)结合的二抗一起孵育后,用含有0.05%的Tween-20的PBS洗板。 (b)每孔加入100ulTMB溶液。孵育特定的时间或者直到产生所需要的信号。请参考说明书中建议的孵育时间。 (c)加入100ul终止液 (d)在450nm处读取吸光值

Background

STOP Solution is used to terminate TMB/peroxidase reaction for ELISA applications and is intended for use in combination with CST's PathScan® Sandwich ELISA Antibody Pairs.

终止液在ELISA中用于终止过氧化物酶/TMB的反应,并且可以与CST公司的PathScan ® Sandwich ELISA Antibody Pairs联合使用。

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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