Cell Signaling Technology

Product Pathways - PathScan ELISA

STOP Solution #7002


No. Size Price
7002L 500 ml ( 50 x 96 well plates ) ¥1,284.00 现货查询 购买询价
7002S 100 ml ( 10 x 96 well plates ) ¥370.00 现货查询 购买询价
7002 carrier free & custom formulation / quantityemail request
Applications Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:


STOP Solution is a proprietary solution used to terminate the peroxidase/TMB reaction for ELISA applications. The TMB substrate reacts with immobilized horseradish peroxidase (HRP) conjugated secondary antibodies to produce a blue solution. Color intensity is an indication of analyte level. After attaining the desired intensity, the reaction is terminated by addition of STOP Solution. Upon addition of STOP Solution the color turns from blue to yellow. Absorbance at 450 nm can be read immediately, and he reaction is stable for one hour.


Directions for Use

(a) After incubation with horseradish peroxidase (HRP) conjugated secondary antibody, wash plates with PBS/0.05% Tween-20. 
 (b) Add 100 μl of TMB solution/well. Incubate for a specific amount of time or until desired signal is achieved. See product datasheet for recommended incubation times for specific antibody pair. 
 (c) Add 100 μl of STOP Solution. 
 (d) Read at Absorbance 450 nm.

(a)与辣根过氧化物酶(HRP)结合的二抗一起孵育后,用含有0.05%的Tween-20的PBS洗板。 (b)每孔加入100ulTMB溶液。孵育特定的时间或者直到产生所需要的信号。请参考说明书中建议的孵育时间。 (c)加入100ul终止液 (d)在450nm处读取吸光值


STOP Solution is used to terminate TMB/peroxidase reaction for ELISA applications and is intended for use in combination with CST's PathScan® Sandwich ELISA Antibody Pairs.

终止液在ELISA中用于终止过氧化物酶/TMB的反应,并且可以与CST公司的PathScan ® Sandwich ELISA Antibody Pairs联合使用。

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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