Cell Signaling Technology

Product Pathways - Protein Stability

UBE2N (D2A1) Rabbit mAb #6999

BLU   E2conjugating   UBC13   UBE2N   ubiquitin  

No. Size Price
6999S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
6999 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 17 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Chicken, Xenopus, Bovine, Dog, Horse,

Specificity / Sensitivity

UBE2N (D2A1)兔单抗识别内源性的UBE2N总蛋白,这一抗体与UBE2D家族成员没有交叉反应。

Source / Purification

UBE2N (D2A1)兔单抗是通过合成对应于人UBE2N蛋白羧基末端序列的肽段,免疫动物获得。 Western blot analysis of extracts from 293T cells, either mock transfected or transfected with a Myc/DDK-tagged cDNA expression construct encoding full-length human UBE2N, UBE2D1, UBE2D2, UBE2D3, or UBE2D4, using UBE2N (D2A1) Rabbit mAb (upper) and DYKDDDDK Tag Antibody (Binds to same epitope as Sigma''s Anti-FLAG® M2 Antibody) #2368 (lower). 使用UBE2N(D2A1)兔单克隆抗体(上)或DYKDDDDK标签抗体(与Sigma FLAG®M2抗体抗原决定簇相同)#2368(下)对,转染对照载体或转染带MYC/DDK标签的UBE2N、UBE2D1、UBE2D2、 UBE2D3或UBE2D4基因全长表达载体的293T细胞提取物免疫印迹分析结果。 Western blot analysis of extracts from various cell lines using UBE2N (D2A1) Rabbit mAb. 使用UBE2N (D2A1)兔单抗对不同细胞裂解物进行免疫印迹分析的结果。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using UBE2N (D2A1) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, either mock transfected or transfected with a Myc/DDK-tagged cDNA expression construct encoding full-length human UBE2N, UBE2D1, UBE2D2, UBE2D3, or UBE2D4, using UBE2N (D2A1) Rabbit mAb (upper) and DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #2368 (lower).

Background

蛋白泛素化是重要的调节蛋白功能与命运的翻译后修饰(1)。泛素化修饰可以通过单泛素化或多泛素化形式偶联到靶蛋白,在泛素分子中有多个赖氨酸残基可以作为多泛素化链的连接位点。不同的多泛素化修饰参与靶蛋白不同的功能,包括改变蛋白功能或导致降解(2)。UBE2N/Ubc13是一个催化K63位点多泛素链形成的泛素E2共价结合酶(1,2)。UBE2N与MMS2或Uev1A形成异源二聚体,发挥其E2泛素共价结合酶功能。UBE2N/MMS2和UBE2N/Uev1A异源二聚体催化不同形式的蛋白泛素化修饰,参与信号通路的调节(3-5),包括:DNA 损伤与重组、p53和细胞周期检验点控制(6-10)、免疫受体相关信号通路(11,12)和细胞内吞过程(13)。特别是最近的研究发现,UBE2N会通过促进K63偶联的泛素化在IL-1β受体信号传递中IKK的下游级联途径等免疫相关信号通路中发挥重要作用(14)。而且, UBE2N与Triad1 E3蛋白泛素酶相互作用,在造血过程中发挥重要作用(15)。

  1. Herrmann, J. et al. (2007) Circ Res 100, 1276-91.
  2. Wilkinson, K.D. et al. (2005) EMBO Rep 6, 815-20.
  3. Hofmann, R.M. and Pickart, C.M. (1999) Cell 96, 645-53.
  4. Deng, L. et al. (2000) Cell 103, 351-61.
  5. Andersen, P.L. et al. (2005) J Cell Biol 170, 745-55.
  6. Zhao, G.Y. et al. (2007) Mol Cell 25, 663-75.
  7. Kolas, N.K. et al. (2007) Science 318, 1637-40.
  8. Laine, A. et al. (2006) Mol Cell Biol 26, 8901-13.
  9. Huen, M.S. et al. (2008) Mol Cell Biol 28, 6104-12.
  10. Loring, G.L. et al. (2008) Cell Cycle 7, 96-105.
  11. Yamamoto, M. et al. (2006) Nat Immunol 7, 962-70.
  12. Yamamoto, M. et al. (2006) J Immunol 177, 7520-4.
  13. Duncan, L.M. et al. (2006) EMBO J 25, 1635-45.
  14. Xu, M. et al. (2009) Mol Cell 36, 302-14.
  15. Marteijn, J.A. et al. (2009) Leukemia 23, 1480-9.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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