Cell Signaling Technology

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Anti-rabbit IgG F(ab')2 Fragment (Sepharose® Bead Conjugate) #6990

No. Size Price
6990S 1 ml ( 40 immunoprecipitations ) ¥1,666.00 现货查询 购买询价
6990 carrier free & custom formulation / quantityemail request
Applications Species-Reactivity Sensitivity MW (kDa) Isotype
IP Endogenous Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: IP=Immunoprecipitation,

Specificity / Sensitivity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, Rabbit IgG and Rabbit serum. No reaction was observed against anti-pepsin, anti-goat IgG Fc, or human or mouse serum proteins.

免疫电泳后出现一条针对抗羊血清、兔IgG和兔血清的沉淀弧,没有出现针对抗胃蛋白酶、抗羊IgGFc或人或小鼠血清蛋白的反应。

Source / Purification

Anti-rabbit IgG F(ab')2 Fragment is produced by immunizing goats with rabbit IgG whole molecules. The Anti-rabbit IgG F(ab')2 Fragment is prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption to remove any unwanted reactivities, pepsin digestion, and chromatographic separation.

该试剂是用兔源IgG全分子免疫羊生产的, Anti-Rabbit IgG F(ab’)2 Fragment 是通过采用兔源IgG偶联琼脂糖微球的免疫亲和色谱法中的单一抗血清制备的,然后采用固相吸附去除不必要的反应如胃蛋白酶消化和色谱分离。

Description

This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Anti-rabbit IgG F(ab') 2 Fragment (Sepharose Bead Conjugate) is useful for the immunoprecipitation of antibodies raised in rabbit.

此种抗体经初级氨基酸团与N-基琥珀酰亚胺酯活化的琼脂糖微球共价结合固定。 Anti-Rabbit IgG F(ab’)2 Fragment (结合琼脂糖微球) 用于兔源抗体的免疫沉淀。

IP

IP

Immunoprecipitation of Jurkat cell extracts using Akt (pan) (C67E7) Rabbit mAb #4691. Extracts were incubated with either Anti-Rabbit IgG F(ab')2 Fragment (Sepharose Bead Conjugate) (lane 1) or Protein A Beads (lane 2). The western blot was probed using Akt (pan) (40D4) Mouse mAb #2920.免疫沉淀分析Jurkat细胞提取物,所用抗体为Akt (pan) (C67E7) Rabbit mAb #4691。提取物与Anti-Rabbit IgG F(ab')2 Fragment (琼脂糖微球结合) (泳道1) 或蛋白A 微球(泳道2).孵育。Western blot采用Akt (pan) (40D4) Mouse mAb #2920进行。

Directions for Use

Add 10-20 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml in 1X Cell Lysis Buffer (10X) #9803. See protocol for more details.

Background

Anti-rabbit IgG F(ab')2 Fragment (Sepharose Bead Conjugate) is ideal for immunoprecipitation. This secondary antibody will detect rabbit antibodies. Since this secondary antibody is made with F(ab')2 fragments, non-specific binding through Fc receptors present on cells will be eliminated.

该试剂适用于免疫沉淀。其二抗可以检测兔源抗体。用F(ab’)2片段制作的二抗,可以减少细胞表面Fc受体的非特异性结合。

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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