Product Pathways - Growth Factors/Cytokines
Mouse His6Interleukin-33 (mHis6IL-33) #6983
|6983LC||50 µg ( With Carrier )||￥7,768.00 现货查询||购买询价|
|6983LF||50 µg ( Carrier Free )||￥7,768.00 现货查询||购买询价|
|6983SC||10 µg ( With Carrier )||￥2,580.00 现货查询||购买询价|
|6983SF||10 µg ( Carrier Free )||￥2,580.00 现货查询||购买询价|
|6983||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Source / Purification
Recombinant mouse His6IL-33 (mHis6IL-33) Ser109-Ile266 (Accession #NP_598536) was produced in E.coli at Cell Signaling Technology.
CST公司利用重组的鼠His6IL-33 (mHis6IL-33) Ser109-Ile266 (Accession #NP_598536)在E.coli细胞中表达。
Recombinant N-terminally His6-tagged mIL-33 has a calculated MW of 19,439. DTT-reduced and non-reduced protein migrate as 22 kDa polypeptides.
>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mHis6IL-33. All lots are greater than 98% pure.
6 μg 还原 (+) 和 非还原的(-) mHis6IL-33通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。
The bioactivity of recombinant mHis6IL-33 was determined by its ability to induce IL-13 production by MC/9 cells. The ED50 of each lot is between 1-6 ng/ml.
The purity of recombinant mHis6IL-33 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mHis6IL-33 and staining overnight with Coomassie Blue.重组蛋白mHis6IL-33的纯度用6 µg 还原(+)和未还原(-)的蛋白走SDS-PAGE 胶来观察。考马斯亮蓝染色过夜。
The production of IL-13 by MC/9 cells cultured with increasing concentrations of mHis6IL-33 was assessed. Media from cells incubated with mHis6IL-33 for 24 hours was collected and assayed for IL-13 production by ELISA and the OD450 was determined.MC/9细胞产生IL-13。细胞在浓度递增的mHis6IL-33中培养。经mHis6IL-33培养24小时后收集培养基并通过ELISA和 OD450值来确定。
Less than 0.01 ng endotoxin/1 μg mHis6IL-33.
内毒素含量:<0.01 ng /1 μg mHis6IL-33。
With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 1 mM EDTA, 3 mM DTT and 20 μg BSA per 1 μg mHis6IL-33. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 1 mM EDTA and 3 mM DTT.
有载体: 每微克mHis6IL-33蛋白溶解在包含1 mM EDTA、3 mM DTT和 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。
无载体：每微克mHis6IL-33蛋白溶解在包含1 mM EDTA 和 3 mM DTT的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。
IL-33 is a novel member of the IL-1 family of proteins that was discovered in a computational search to identify unknown family members (1). IL-33 is expressed by fibroblasts and endothelial cells, and is upregulated in response to inflammatory stimuli (1). IL-33 plays a key role in Th2 type inflammatory responses, including atopic allergic responses and asthma (1,2). The IL-33 receptor consists of the ST2 and IL-1 accessory protein (IL-1-Acp) (1,3). ST2 is expressed on mast cells, basophils and Th2 cells (1). In addition to its putative role as a Th2 cytokine, IL-33 also contains a nuclear localization domain and may function as a nuclear factor (4,5). The nuclear function of IL-33 remains unclear. IL-33 is released by cellular necrosis, similar to other “alarmins”, thereby signaling tissue injury (5,6). However, more recent data suggests that IL-33 may also be actively secreted from live cells via a non-classical mechanism (7).
IL-33是IL-1蛋白家族中新的成员，此蛋白是在利用计算机技术鉴定未知家族成员时发现的(1)。IL-33 在纤维原和上皮细胞中表达, 并在炎症刺激中上调表达(1)。IL-33在Th2类型的炎症应答中发挥了重要的作用, 包括遗传性过敏症反应和哮喘(1,2)。IL-33受体由ST2 和 IL-1附属蛋白组成(IL-1-Acp) (1,3)。ST2在柱状细胞、嗜碱细胞和Th2细胞上表达(1)。除了作为Th2细胞因子的潜在功能外，IL-33也具有细胞核定位域并有可能具有细胞核因子的功能(4,5)。IL-33的细胞核功能依然不清楚。IL-33与其它的“报警因子”相似，通过细胞坏死而释放,从而向周围受伤组织发出信号(5,6)。然而，近来越来越多的数据表明IL-33也能通过非经典机制从活细胞中有活性的分泌出来(7)。
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
用户评论 --- 共 0 条