Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-Rad17 (Ser645) (D5H5) Rabbit mAb #6981

No. Size Price
6981S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
6981 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 80 Rabbit IgG
IP 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-Rad17 (Ser645) (D5H5) Rabbit mAb recognizes endogenous levels of Rad17 protein only when phosphorylated at Ser645.Phospho-Rad17 (Ser645) (D5H5) Rabbit mAb 能够检测内源性丝氨酸(645位)磷酸化的Rad17蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser645 of human Rad17 protein.该单克隆抗体是由合成的人源的针对Rad17蛋白丝氨酸(645位)的肽段免疫动物生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or treated with CIP and λ phosphatase, using Phospho-Rad17 (Ser645) (D5H5) Rabbit mAb (upper) or Rad17 (D3G6) Rabbit mAb #8561 (lower).Western blot方法检测Hela细胞,分为非处理组和CIP与λ磷酸酶共处理组,使用的抗体为Phospho-Rad17 (Ser645) (D5H5) Rabbit mAb (上图) 或 Rad17 (D3G6) Rabbit mAb #8561 (下图)。

Western Blotting

Western Blotting

Western blot analysis of extracts from MRC-5 cells, untreated or UV-treated (100 mJ/cm2, 2 hr recovery), using Phospho-Rad17 (Ser645) (D5H5) Rabbit mAb.Western blot方法检测MRC-5细胞提取物,分为非处理组和紫外处理组(100 mJ/cm2, 恢复2小时), 使用的抗体为Phospho-Rad17 (Ser645) (D5H5) Rabbit mAb。

IP

IP

Immunoprecipitation of phosphorylated Rad17 from MRC-5 cells, untreated or UV-treated (100 mJ/cm2, 2 hr recovery), using Phospho-Rad17 (Ser645) (D5H5) Rabbit mAb. Western blot was performed using the same antibody. Lanes 1 and 3 are 10% input.从MRC-5细胞免疫沉淀的磷酸化Rad17蛋白,分为非处理组和紫外处理组 (100 mJ/cm2, 恢复2小时),使用的抗体为 Phospho-Rad17 (Ser645) (D5H5) Rabbit mAb。Western blot使用同一抗体,泳道1和3为10%input。

Background

The human checkpoint protein Rad17 and its fission and budding yeast orthologues (Schizosaccharomyces pombe Rad17 and Saccharomyces cerevisiae Rad24, respectively) are involved in the activation of checkpoint signals in response to DNA damage or disruption of DNA synthesis (1-4). Treatment of human cells with genotoxic agents induces ATM/ATR-dependent phosphorylation of Rad17 at Ser635 and Ser645. Rad17 phosphorylation is a critical early event during checkpoint signaling in DNA-damaged cells (5-7).人的检验点蛋白Rad17和其裂变和芽殖酵母的同源基因(裂殖酵母和酿酒酵母中分别为Rad17和Rad24)参与响应DNA损伤或DNA合成破坏的检验点信号激活(1-4)。基因毒性药物处理人体细胞能够诱导ATM/ATR依赖的RAD17的Ser635和Ser645磷酸化。Rad17磷酸化在DNA损伤细胞的检验点信号中是一个重要的早期事件(5-7)。

  1. Griffiths, D.J. et al. (1995) EMBO J 14, 5812-23.
  2. Li, L. et al. (1999) Oncogene 18, 1689-99.
  3. Bao, S. et al. (1998) Cell Growth Differ 9, 961-7.
  4. von Deimling, F. et al. (1999) Hum Genet 105, 17-27.
  5. Bao, S. et al. (2001) Nature 411, 969-74.
  6. Post, S. et al. (2001) Proc Natl Acad Sci U S A 98, 13102-7.
  7. Wang, X. et al. (2001) Cancer Res 61, 7417-21.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications