Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-PKC Substrate Motif [(R/KXpSX(R/K)] MultiMab™ Rabbit mAb mix #6967

KinomeView   Motif Antibody   PhosphoScan   PTMScan  

No. Size Price
6967S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
6967 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 All Species Expected, Endogenous Rabbit IgG
IP 1:50
E-P 1:1000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, E-P=Peptide ELISA (DELFIA),

Specificity / Sensitivity

Phospho-(Ser) PKC Substrate (P-S3-101) Rabbit mAb detects endogenous levels of many cellular proteins only when phosphorylated at Ser residues surrounded by Arg or Lys at the -2 and +2 positions. The antibody may also cross-react with S*X(R/K) motif-containing proteins. The antibody does not cross-react with nonphosphorylated Ser residues, phospho-Thr in the same motif, or phospho-Ser in other motifs.

Phospho-(Ser) PKC Substrate (P-S3-101) Rabbit mAb可识别内源性的+2和-2位是精氨酸或赖氨酸的磷酸化丝氨酸。此抗体可能与包含S*X(R/K)基序的蛋白有交叉反应。该抗体不与非磷酸化丝氨酸残基、同样基序中磷酸化的苏氨酸残基、其它基序中的磷酸化丝氨酸残基发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic phospho-(Ser) PKC substrate peptides. The antibody is formulated from three rabbit monoclones in order to cover a broad range of reactivity.

该单克隆抗体用合成的磷酸化丝氨酸PKC底物免疫动物制备。该抗体是两个兔单克隆的混合,以便可以覆盖更大的反应范围。

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated (-) or TPA-treated (200 nM, 30 min; +), using Phospho-(Ser) PKC Substrate (P-S3-101) Rabbit mAb. Western blot was imaged using Odyssey® Infrared Imaging System (LI-COR® Biotechnologies).

对未处理(-)或200nM TPA处理30分钟(+)的Jurkat细胞抽提液使用Phospho-(Ser) PKC Substrate (P-S3-101) Rabbit mAb进行Western blot分析。Western Blot图片采集使用Odyssey® Infrared Imaging System (LI-COR® Biotechnologies)。

IP

IP

Immunoprecipitation (IP) of extracts from Jurkat cells, untreated (-) or TPA-treated (200 nM, 30 min; +) (lanes 3 and 4), using Phospho-(Ser) PKC Substrate (P-S3-101) Rabbit mAb. 10% input is shown in lanes 1 and 2. Western blot analysis was performed using the same antibody.

对未处理(-)或200nM TPA处理30分钟的(+)(列3、4)的Jurkat细胞,使用Phospho-(Ser) PKC Substrate (P-S3-101) Rabbit mAb兔单抗进行免疫沉淀。10%输入对照显示在列1和2。Western Blot分析用相同抗体进行。

Western Blotting

Western Blotting

Western blot analysis of extracts from various mouse tissues using Phospho-(Ser) PKC Substrate (P-S3-101) Rabbit mAb.

对多种小鼠组织抽提液使用Phospho-(Ser) PKC Substrate (P-S3-101) Rabbit mAb兔单抗进行Western blot分析。

Background

Although protein kinase C (PKC) family members are involved in a number of signal transduction processes including secretion, gene expression, proliferation, and muscle contraction (1,2), many of their substrates remain unidentified. Isozymes of PKC are subdivided into conventional PKCs (cPKC), novel PKCs (nPKC), and atypical PKCs (aPKC). PKCα, βI, βII, and γ isoforms belong to the cPKC group (1). When activated, cPKC isozymes phosphorylate substrates containing Ser or Thr, with Arg or Lys at the -3, -2, and +2 positions, and a hydrophobic amino acid at position +1 (1-3). To identify potential PKC substrates, Cell Signaling Technology has developed antibodies that recognize this motif. Phospho-(Ser) PKC Substrate (D13C5/D47H10/D50E3) Rabbit mAb is specific to cPKC substrates containing phospho-Ser.

尽管蛋白激酶C(PKC)家族成员参与多种信号转导过程,包括分泌、基因表达、增殖和肌肉收缩(1,2),但它们的很多底物仍然未确定。PKC的同工酶分为传统型(cPKC),新型PKC(nPKC)和非典型PKC(aPKC)。PKCα, βI, βII, 和γ属于cPKC组(1)。激活后cPKC同工酶磷酸化精氨酸或赖氨酸在-3,-2和+2位,和+1位是疏水氨基酸的丝氨酸和苏氨酸(1-3)。为了鉴别潜在的PKC底物,Cell Signaling Technology公司开发了识别此基序的抗体。Phospho-(Ser) PKC Substrate (D13C5/D47H10/D50E3) Rabbit mAb兔单抗特异性识别包含磷酸化丝氨酸的cPKC底物。

  1. Nishikawa, K. et al. (1997) J Biol Chem 272, 952-60.
  2. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
  3. Obata, T. et al. (2000) J Biol Chem 275, 36108-15.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

LI-COR is a registered trademark of LI-COR, Inc.

Odyssey is a registered trademark of LI-COR, Inc.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

MultiMab is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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