Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Mis18-α (D3N2X) Rabbit mAb #69625

No. Size Price
69625S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
69625 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 26 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Mis18-alpha (D3N2X) Rabbit mAb recognizes endogenous levels of total Mis18-alpha protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg41 of human Mis18-alpha protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Mis18-alpha (D3N2X) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected (+) with full length human Mis18-alpha/Myc-DDK, using Mis18-alpha (D3N2X) Rabbit mAb (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower).

Background

CENP-A, also known as the chromatin-associated protein CSE4 (capping-enzyme suppressor 4-p), is an essential histone H3 variant that replaces canonical histone H3 in centromeric heterochromatin. The inherited localization of the centromere is specified by CENP-A (1). CENP-A deposition to the correct chromosomal location in early G1 phase is regulated by the Mis18 complex, which consists of Mis18-alpha, Mis18-beta, Mis18BP1, RbAp48 and RbAp46 (2).

Mis18-alpha deficiency in mice results in inappropriate localization of CENP-A, as well as DNA methylation defects (3). Localization of the Mis18 complex to centromeres is regulated by the mitotic kinase Plk1 (polo-like kinase 1) (4).

  1. Ausió, J. (2006) Brief Funct Genomic Proteomic 5, 228-43.
  2. Stellfox, M.E. et al. (2013) Cell Mol Life Sci 70, 387-406.
  3. Kim, I.S. et al. (2012) Mol Cell 46, 260-73.
  4. McKinley, K.L. and Cheeseman, I.M. (2014) Cell 158, 397-411.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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