Cell Signaling Technology

Product Pathways - Motif Antibodies

Acetylated-Lysine (Ac-K-100) MultiMab™ Rabbit mAb mix (HRP Conjugate) #6952

acetyl lysine   acetylated lysine   acetylation   motif  

No. Size Price
6952S 100 µl ( 10 western blots ) ¥3,986.00 现货查询 购买询价
6952 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Monkey,All Species Expected, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Acetylated-Lysine (Ac-K2-100) Rabbit mAb (HRP Conjugate) detects proteins post-translationally modified by acetylation on the ε-amine groups of lysine residues. The antibody recognizes acetylated lysine in a wide range of sequence contexts. It has been demonstrated to recognize acetylated histones, p53, CBP, PCAF, and chemically acetylated BSA. The antibody has been shown to react with as little as 0.04 ng of chemically acetylated BSA while not recognizing up to 25 µg of non-acetylated BSA. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Acetylated-Lysine (Ac-K2-100) Rabbit mAb (HRP Conjugate)HRP偶联兔单抗识别赖氨酸残基ε-氨基上乙酰化蛋白翻译后修饰的蛋白质。此抗体可识别不同序列背景中的乙酰化赖氨酸。研究显示它可以识别乙酰化的组蛋白、p53、CBP、PCAF和化学乙酰化的BSA。抗体可以与少到0.04ng的化学乙酰化的BSA反应,却不能与非乙酰化的多到25ug的BSA反应。(美国专利号:6,441,140、6,982,318、7,259,022、7,344,714;U.S.S.N. 11,484,485;及所有国外相应专利)

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic acetyl-lysine peptide library. The antibody is formulated from two rabbit monoclones in order to cover broad range of reactivity.

该单克隆抗体用合成的乙酰化赖氨酸底物库肽段免疫动物制备。该抗体是两个兔单克隆群的混合物,以便可以覆盖更大的反应范围。

Description

This Cell Signaling Technology® antibody is conjugated by the covalent reaction of hydrazinonicotinamide-modified antibody with formylbenzamide-modified horseradish peroxidase (HRP). The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Acetylated-Lysine (Ac-K2-100) Rabbit mAb #9814.

CST生产的此抗体通过共价结合肼基烟酰胺修饰的抗体与甲酰苯甲酰胺修饰的磁珠而固定。HRP结合的抗体预计可以与非结合的Acetylated-Lysine (Ac-K2-100) Rabbit mAb #9814有同样的交叉反应活性。

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7 cells, untreated or treated with Trichostatin A (TSA) #9950 (1 µM, 6 hr), using Acetylated-Lysine (Ac-K2-100) Rabbit mAb (HRP Conjugate).

对未处理或1uM Trichostatin A (TSA) #9950处理6小时的MCF7细胞抽提液使用Acetylated-Lysine (Ac-K2-100) Rabbit mAb (HRP Conjugate)进行Western blot分析。

ELISA-Peptide

ELISA-Peptide

Signal-to-noise (S/N) values of acetylated versus non-acetylated peptides analyzed using Acetylated-Lysine (Ac-K2-100) Rabbit mAb (HRP Conjugate) and 20X LumiGLO® Reagent and 20X Peroxide #7003.

使用Acetylated-Lysine (Ac-K2-100) Rabbit mAb (HRP Conjugate)和20X LumiGLO® Reagent和20X Peroxide #7003得到的乙酰化肽段和非乙酰化肽段的信噪比。

Background

Acetylation of lysine, like phosphorylation of serine, threonine or tyrosine, is an important reversible modification controlling protein activity. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (1). Signaling resulting in acetylation/deacetylation of histones, transcription factors, and other proteins affects a diverse array of cellular processes including chromatin structure and gene activity, cell growth, differentiation, and apoptosis (2-6). Recent proteomic surveys suggest that acetylation of lysine residues may be a widespread and important form of posttranslational protein modification that affects thousands of proteins involved in control of cell cycle and metabolism, longevity, actin polymerization, and nuclear transport (7,8). The regulation of protein acetylation status is impaired in cancer and polyglutamine diseases (9), and HDACs have become promising targets for anti-cancer drugs currently in development (10).

赖氨酸的乙酰化如同丝氨酸、苏氨酸和酪氨酸的磷酸化一样,是一种控制蛋白活性的重要可逆手段。四个核心组蛋白(H2A, H2B, H3, H4)的保守氨基末端区域均包含赖氨酸,可以被组蛋白乙酰转移酶(HATs)乙酰化和被组蛋白脱乙酰酶(HDACs)去乙酰化(1)。组蛋白、转录因子和其它影响细胞内染色体结构、基因活性、细胞生长、分化和凋亡的蛋白都经乙酰化/去乙酰化的信号影响(2-6)。最近的蛋白组学研究显示赖氨酸残基的乙酰化是蛋白翻译后修饰的广泛而重要的手段,可以影响控制细胞周期、代谢、生存、肌动蛋白聚合和核转运相关的数千种蛋白。蛋白质乙酰化调节障碍在癌症和多聚谷氨酰胺疾病中发现(9),HDACs也成为目前正在开发的有希望的抗癌药物靶点(10)。

  1. Hassig, C.A. and Schreiber, S.L. (1997) Curr Opin Chem Biol 1, 300-8.
  2. Allfrey, V.G. et al. (1964) Proc Natl Acad Sci USA 51, 786-94.
  3. Liu, L. et al. (1999) Mol Cell Biol 19, 1202-9.
  4. Boyes, J. et al. (1998) Nature 396, 594-8.
  5. Polevoda, B. and Sherman, F. (2002) Genome Biol 3, reviews 0006.
  6. Yoshida, M. et al. (2003) Prog Cell Cycle Res 5, 269-78.
  7. Kim, S.C. et al. (2006) Mol Cell 23, 607-18.
  8. Choudhary, C. et al. (2009) Science 325, 834-40.
  9. Hughes, R.E. (2002) Curr Biol 12, R141-3.
  10. Vigushin, D.M. and Coombes, R.C. (2004) Curr Cancer Drug Targets 4, 205-18.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.

MultiMab is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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