Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (HRP Conjugate) #6950

AKT   akt1   akt2   akt3   general   motif   rabbit monoclonal   RXRXXS   RXRXXT   substrate  

No. Size Price
6950S 100 µl ( 10 western blots ) ¥3,986.00 现货查询 购买询价
6950 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse,D. melanogaster,All Species Expected, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (HRP Conjugate) recognizes peptides and proteins containing phospho-serine/threonine preceded by arginine at the -3 position. There is some preference observed for peptides that contain phospho-serine/threonine preceded by arginine at both positions -5 and -3.

Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (HRP Conjugate)识别包含-3位上有精氨酸的磷酸化丝氨酸/苏氨酸的肽段和蛋白。此抗体与包含-5、-3位上有精氨酸的磷酸化丝氨酸/苏氨酸的肽段有部分的交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic phospho-Akt substrate peptides.



This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb #9614.

CST公司生产的抗体与辣根过氧化物酶(HRP)的碳水化合物基团通过其氨基基团相结合。HRP结合的抗体预计可以与非结合的Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb #9614有同种交叉反应。

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat and HeLa cells, untreated or treated with Calyculin A #9902 (0.1 µM, 45 min), using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (HRP Conjugate).

对未处理或0.1uM Calyculin A #9902裂解前处理45分钟的Jurkat和HeLa细胞抽提液使用Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (HRP Conjugate)进行Western blot分析。


An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation (3-5). While a number of Akt substrates are known (such as GSK-3, Bad, and caspase-9) many important substrates await discovery. Akt phosphorylates substrates only at Ser/Thr in a conserved motif characterized by Arg at positions -5 and -3 (6). Phospho-Akt substrate-specific antibodies from CST are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery.

精氨酸导向的激酶或AGC家族激酶是很重要的激酶,包括cAMP依赖的蛋白激酶(PKA)、cGMP依赖的蛋白激酶(PKG)、蛋白激酶C、Akt和RSK。这些激酶有相同的底物特异性,-3位上有精氨酸,与磷酸化的丝氨酸和苏氨酸相关(1,2)。Akt在介导细胞内关键反应中扮演重要角色,包括细胞生长和生存、血管生成和转录调节(3-5)。目前已经发现一些Akt底物(如GSK-3、Bad、caspase-9),但还有很多重要底物等待发现。Akt磷酸化保守序列中精氨酸在-5、-3位置的丝氨酸/苏氨酸(6)。磷酸化Akt底物特异性抗体由Cell Signaling Technology公司提供,用于研究Akt磷酸化调节和精氨酸导向的激酶,也可以用于高通量药物发现。

  1. Montminy, M. (1997) Annu Rev Biochem 66, 807-22.
  2. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
  3. Marte, B.M. and Downward, J. (1997) Trends Biochem Sci 22, 355-8.
  4. Jiang, B.H. et al. (2000) Proc Natl Acad Sci USA 97, 1749-53.
  5. Scheid, M.P. and Woodgett, J.R. (2000) Curr Biol 10, R191-4.
  6. Alessi, D.R. et al. (1996) FEBS Lett 399, 333-8.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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