Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-Threonine Antibody (P-Thr-Polyclonal) (HRP Conjugate) #6949

general   motif   phosphothreonine   substrate  

No. Size Price
6949S 100 µl ( 10 western blots ) ¥3,986.00 现货查询 购买询价
6949 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous Rabbit
E-P 1:2000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, E-P=Peptide ELISA (DELFIA),

Specificity / Sensitivity

Phospho-Threonine Antibody (P-Thr-Polyclonal) (HRP Conjugate) detects proteins and peptides phosphorylated at threonine residues in a manner largely independent of the surrounding amino acid sequence. The antibody is phospho-specific and may cross-react with some phospho-serine-containing sequences. By ELISA, it recognizes a wide variety of threonine-phosphorylated peptides, and by 2D gel western blot analysis, it recognizes a large number of presumably threonine-phosphorylated proteins. We recommend the use of Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101) #9391 to detect proteins containing threonine followed by proline. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Phospho-Threonine Antibody (P-Thr-Polyclonal) (HRP Conjugate)可以识别苏氨酸残基磷酸化的蛋白质和肽段,与周围氨基酸序列磷酸化情况无关。该抗体是磷酸化特异性的,可能与其它包含磷酸化丝氨酸的序列发生交叉反应。在ELISA中,其可以识别多种苏氨酸磷酸化的肽段,在二维凝胶Western Blot中,其可以识别很多推测的苏氨酸磷酸化的蛋白。CST推荐使用Phospho-Threonine-Proline mAb (p-Thr-Pro-101) #9391来检测包含脯氨酸之前是苏氨酸的蛋白质。(美国专利号:6,441,140、6,982,318、7,259,022、7,344,714;U.S.S.N. 11,484,485;及所有国外相应专利)

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic phospho-Thr-containing peptides . Antibodies are purified by protein A and peptide affinity chromatography.该多克隆抗体用合成的包含磷酸化苏氨酸的肽段免疫动物制备。该抗体使用蛋白A和蛋白亲和层析纯化。

Description

This Cell Signaling Technology® antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Threonine Antibody (P-Thr-Polyclonal) #9381.

CST公司生产的抗体与辣根过氧化物酶(HRP)的碳水化合物基团通过其氨基基团相结合。HRP偶联的抗体可以与未偶联的Phospho-Threonine Antibody (P-Thr-Polyclonal) #9381产生相同的交叉反应活性。

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated or treated with Calyculin A #9902 (0.1 µM for 45 minutes), using Phospho-Threonine Antibody (P-Thr-Polyclonal) (HRP Conjugate).

对未处理或0.1uM calyculin A裂解前处理45分钟的Jurkat细胞抽提液使用Phospho-Threonine Antibody (P-Thr-Polyclonal) (HRP Conjugate)进行Western blot分析。

ELISA-Peptide

ELISA-Peptide

Signal-to-noise (S/N) values of phosphopeptide versus nonphosphopeptide signals using LumiGLO® and Peroxide Reagents #7003.

使用LumiGLO® and Peroxide Reagents #7003的磷酸化肽段和非磷酸化肽段的信噪比。

Background

Much of the dynamic behavior of cellular proteins, including the regulation of molecular interactions (1), subcellular localization (2), and transcriptional regulation (3) is controlled by a variety of post-translational modifications (4). Antibodies specific for these post-translational modifications are invaluable tools in the quest to understand normal and pathogenic molecular and cellular behavior. 
 General protein modification antibodies are designed to react with modified amino acid residues (e.g., phospho-threonine, phospho-tyrosine, acetyl-lysine, nitro-tyrosine) independently of the sequence in which they are embedded. This ability to recognize modified residues in a "context independent" fashion gives these antibodies broad reactivities, presumably conferring upon them the ability to react with hundreds of distinct proteins. This broad pattern of reactivity makes these antibodies especially valuable in multiplex analyses and target discovery programs.

细胞内很多蛋白质的动态行为,包括分子相互作用调节(1),亚细胞定位(2)和转录调节(3)都由多种转录后修饰调控(4)。这些转录后修饰特异性的抗体对理解正常和病理的分子和细胞行为非常有价值。大部分蛋白修饰抗体设计出来与修饰的氨基酸残基发生反应(如磷酸化苏氨酸、磷酸化酪氨酸、乙酰化赖氨酸、硝基化酪氨酸),而不考虑这些残疾在序列中的位置。这种不考虑上下文而仅识别修饰后残基的能力给予这些抗体广泛的识别可能,赋予其识别上百种不同蛋白质的能力。这种广泛的识别特征使这些抗体在复杂分析和目标筛查的过程中具有特别重要的价值。

  1. Yaffe, M.B. and Elia, A.E. (2001) Curr Opin Cell Biol 13, 131-8.
  2. Appella, E. and Anderson, C.W. (2001) Eur J Biochem 268, 2764-72.
  3. Jenuwein, T. and Allis, C.D. (2001) Science 293, 1074-80.
  4. Krishna, R.G. and Wold, F. (1993) Adv Enzymol Relat Areas Mol Biol 67, 265-98.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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