Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb #6943

fyn   hck   lck   lyn   sc-12958   tck   yes   yrk  

No. Size Price
6943S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
6943T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价 防伪查询
6943 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 60 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-Src Family (Tyr416) (D49G8) Rabbit mAb detects endogenous levels of Src only when phosphorylated at Tyr416. The antibody may cross-react with other Src family members (Lyn, Fyn, Lck, Yes and Hck) when phosphorylated at equivalent sites. It may cross react with overexpressed phosphorylated RTKs.

Phospho-Src Family (Tyr416) (D49G8) Rabbit mAb兔单抗能够检测内源性的Tyr416位点磷酸化的Src蛋白。该抗体可能会与相同位点被磷酸化的其他Src家族成员(Lyn, Fyn, Lck, Yes和Hck)发生交叉反应。该抗体可能会与过表达的磷酸化RTKs发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr416 of human Src protein.


Western Blotting

Western Blotting

Western blot analysis of extracts NIH/3T3 cells, serum-starved or treated with human Platelet-Derived Growth Factor BB hPDGF-BB #8912 (100 ng/ml, 15 min), using Phospho-Src Family (Tyr416) (D49G8) Rabbit mAb (upper) or Src (36D10) Rabbit mAb #2109 (lower).

采用Phospho-Src Family (Tyr416) (D49G8) Rabbit mAb兔单抗 (上图)和Src (36D10) Rabbit mAb兔单抗 #2109 (下图),对饥饿培养的NIH/3T3细胞和人血小板衍生生长因子BB hPDGF-BB #8912 (100 ng/ml, 15 min)处理的NIH/3T3细胞提取物进行Western blot分析。


The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).

Src家族蛋白酪氨酸激酶包括Src, Lyn, Fyn, Yes, Lck, Blk, Hck, 对调节真核细胞的生长和分化很重要(1)。Src的活性是通过两个酪氨酸位点的磷酸化来调控的,但是这两者的调控效应相反。激酶结构域的活化环中的Tyr416位点的磷酸化,能够上调酶活性;而蛋白羧基端尾部Tyr527位点被Csk磷酸化后,则会使酶的活性降低(2)。

  1. Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609.
  2. Hunter, T. (1987) Cell 49, 1-4.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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