Cell Signaling Technology

Product Pathways - Metabolism

Phospho-TBC1D1 (Ser700) Antibody #6929

KIAA1108   TBC   TBC1   TBC1 domain family member 1   TBC1D1   TBCD1  

No. Size Price
6929S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
6929 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse, Transfected Only 160 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Rat,

Specificity / Sensitivity

Phospho-TBC1D1 (Ser700) Antibody detects transfected levels of TBC1D1 protein when phosphorylated at Ser700.

Phospho-TBC1D1 (Ser700) Antibody用于检测在转染的700位丝氨酸磷酸化的TBC1D1蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser700 of mouse TBC1D1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体是通过合成对应于鼠TBC1D1蛋白Ser700及邻近序列的肽段免疫动物获得,抗体经Protein A和肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, untransfected or transfected with mouse wild-type TBC1D1 or mutant TBC1D1 (S700A), using Phospho-TBC1D1 (Ser700) Antibody (upper panel) or TBC1D1 (G689) Antibody #5929 (lower panel). Both of the above expression vectors were kindly provided by Dr. Laurie Goodyear at the Joslin Diabetes Center.

Western blot检测293T细胞提取物,细胞用鼠野生型TBC1D1和突变TBC1D1(S700A)进行了转染,所用抗体为Phospho-TBC1D1 (Ser700) Antibody(上)和TBC1D1 (G689) Antibody #5929(下)。两个表达载体都由Joslin糖尿病中心的Laurie Goodyear博士馈赠。

Background

TBC1D1 is a paralog of AS160 (1) and both proteins share about 50% identity (2). TBC1D1 was shown to be a candidate gene for severe obesity (3). It plays a role in Glut4 translocation through its GAP activity (2,4). Studies indicate that TBC1D1 is highly expressed in skeletal muscle (1). Insulin, AICAR, and contraction directly regulate TBC1D1 phosphorylation in this tissue (1). Three AMPK phosphorylation sites (Ser231, Ser660, and Ser700) and one Akt phosphorylation site (Thr590) were identified (5). Muscle contraction or AICAR treatment increases phosphorylation on Ser231, Ser660, and Ser700 but not on Thr590; insulin increases phosphorylation on Thr590 only (5).

TBC1D1是AS160(1)的旁系同源蛋白,他们有着约50%的相似性(2)。TBC1D1可能是严重肥胖的一个致病基因(3)。它通过自身的GAP活性在Glut4转移过程中发挥作用(2,4)。研究表明,TBC1D1在骨胳肌中高度表达(1)。胰岛素,AICAR以及它的浓度可以调节TBC1D1在骨骼肌中的磷酸化(1)。已经发现,TBC1D1中有三个AMPK磷酸化位点(Ser231, Ser660, 和Ser700)和一个Akt磷酸化位点(Thr590)(5)。肌肉收缩和AICAR处理会增加Ser231, Ser660, 和Ser700的磷酸化水平,但不会影响Thr590。只有胰岛素会增加Thr590位点的磷酸化水平(5)。

  1. Taylor, E.B. et al. (2008) J Biol Chem 283, 9787-96.
  2. Roach, W.G. et al. (2007) Biochem J 403, 353-8.
  3. Stone, S. et al. (2006) Hum Mol Genet 15, 2709-20.
  4. Chavez, J.A. et al. (2008) J Biol Chem 283, 9187-95.
  5. Vichaiwong, K. et al. (2010) Biochem J 431, 311-20.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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