Cell Signaling Technology

Product Pathways - Autophagy Signaling

Phospho-ULK1 (Ser757) Antibody #6888

No. Size Price
6888S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
6888T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
6888 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Monkey, Endogenous 140-150 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-ULK1 (Ser757) Antibody recognizes endogenous levels of ULK1 protein only when phosphorylated at Ser757.

Phospho-ULK1 (Ser757) Antibody 能够检测内源性的仅Ser757发生磷酸化的ULK1蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser757 of human ULK1 protein. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from A-431 cells, untreated or treated with Human Epidermal Growth Factor (hEGF) #8916 (100 ng/ml, 30 min) using Phospho-ULK1 (Ser757) Antibody (upper), or α-Tubulin (11H10) Rabbit mAb #2125 (lower).

Western blot 分析A-431细胞的细胞提取物,未处理或人表皮生长因子 (hEGF) #8916 处理 (100 ng/ml, 30 min) ,使用抗体是Phospho-ULK1 (Ser757) Antibody (上图)或 α-Tubulin (11H10) Rabbit mAb 兔单抗#2125 (下图).

Western Blotting

Western Blotting

Western blot analysis of extracts from A172 cells, untreated (-), Torin 1-treated (+; 250 nM; 5 hrs), or INK-128-treated (+; 250 nM; 5 hrs) using Phospho-ULK1 (Ser757) Antibody (upper) or total ULK1 (D8H5) Rabbit mAb #8054 (lower).


Two related serine/threonine kinases, UNC-51-like kinase -1 and -2 (ULK1, ULK2), were discovered as mammalian homologs of the C. elegans gene UNC-51 in which mutants exhibited abnormal axonal extension and growth (1-4). Both proteins are widely expressed and contain an amino-terminal kinase domain followed by a central proline/serine rich domain and a highly conserved carboxy-terminal domain. The roles of ULK1 and ULK2 in axon growth have been linked to studies showing that the kinases are localized to neuronal growth cones and are involved in endocytosis of critical growth factors such as NGF (5). Yeast two-hybrid studies found ULK1/2 associated with modulators of the endocytic pathway, SynGap, and syntenin (6). Structural similarity of ULK1/2 has also been recognized with the yeast autophagy protein Atg1/Apg1 (7). Knockdown experiments using siRNA demonstrated that ULK1 is essential for autophagy (8), a catabolic process for the degradation of bulk cytoplasmic contents (9,10). It appears that Atg1/ULK1 can act as a convergence point for multiple signals that control autophagy (11), and can bind to several autophagy-related (Atg) proteins, regulating phosphorylation states and protein trafficking (12-16).

UNC-51样激酶1和2(ULK1, ULK2),是两种相关丝氨酸/苏氨酸激酶,研究发现是秀丽隐杆线虫基因UNC-51的哺乳动物同源物,它的突变会导致轴突的异常延伸与生长(1-4)。两种蛋白广泛表达,并且在一个中央脯氨酸/丝氨酸富含区域,和一个高度保守的羧基末端区域的后面含有一个氨基末端激酶域。关于ULK1和ULK2在轴突生长中的作用,有关研究表明,这些激酶主要定位于神经生长锥中,并且参与临界生长因子的内吞作用,如NGF(5)。酵母双杂交试验研究发现,ULK1/2与内吞作用途径的调节器有关,如SynGAP 和 syntenin(6)。ULK1/2与酵母自噬蛋白Agt1/Apg1具有结构相似性(7)。使用siRNA的敲除实验证明了,ULK1对自噬是必不可少的,即自噬溶酶体对其包裹的细胞质内容物进行降解的一种分解代谢过程(8)。Atg1/ULK1能够作为控制通路的多重信号的聚焦点(11),并且能够与多种调节磷酸化状态和蛋白运输的自噬相关蛋白(Atg)相结合(12-16)。

AMPK, activated during low nutrient conditions, directly phophorylates ULK1 at muliptle sites including Ser317, Ser555, and Ser777 (17, 18). Conversely, mTOR, which is a regulator of cell growth and is an inhibitor of autophagy, phosphorylates ULK1 at Ser757 and disrupts the interaction between ULK1 and AMPK (17).

AMPK,在低营养条件下的活化,可以直接磷酸化ULK1的多个位点,如Ser317、Ser555 和 Ser777 (17,18)。相反,mTOR,能够调节细胞生长和自噬抑制物,可以磷酸化ULK1的Ser757位点,并破坏ULK1和AMPK的相互作用(17)。

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  16. Hara, T. et al. (2008) J Cell Biol 181, 497-510.
  17. Kim, J. et al. (2011) Nat Cell Biol 13, 132-41.
  18. Egan, D.F. et al. (2011) Science 331, 456-61.

Application References

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