Cell Signaling Technology

Product Pathways - Tag Antibodies

HPC4-Tag Antibody #68083

No. Size Price
68083S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
68083 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 All Species Expected, Transfected Only Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

HPC4-Tag Antibody recognizes recombinant proteins containing the HPC4-tag. This antibody recognizes the HPC4-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues of the HPC4-tag.



Immunoprecipitation of transfected HPC4-tagged GFP fusion protein from 293T cell extracts. Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 is HPC4-Tag Antibody. Western blot analysis was performed using HPC4-Tag Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, untransfected (-) or transfected with amino-terminal or carboxy-terminal HPC4-tagged GFP fusion protein (+), using HPC4-Tag Antibody.


Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

HPC4-tag is a 12 amino acid sequence derived from protein C, which is a vitamin K-dependent plasma zymogen. Protein C is activated by proteolytic cleavage of the thrombin-thrombomodulin complex to form an anticoagulant enzyme (1,2). This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (3,4).

  1. Kurosawa, S. (1989) Nihon Ketsueki Gakkai Zasshi 52, 1343-9.
  2. Rezaie, A.R. and Esmon, C.T. (1992) J Biol Chem 267, 26104-9.
  3. Tie, J.K. et al. (2005) J Biol Chem 280, 16410-6.
  4. Chu, P.H. et al. (2006) Proc Natl Acad Sci U S A 103, 19308-13.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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