Product Pathways - Innate Immunity
GBP5 (D3A5O) Rabbit mAb #67798
|67798S||100 µl ( 10 western blots )||￥3,250.00 现货查询||购买询价|
|67798||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation,
Specificity / Sensitivity
GBP5 (D3A5O) Rabbit mAb recognizes endogenous levels of total GBP5 protein. Depending on Western blotting conditions, GBP5 is sometimes observed as a doublet. This is likely due to cleavage of a predicted three amino acid propeptide sequence.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala561 of human GBP5 protein.
Western blot analysis of extracts from THP-1 cells, differentiated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (80 nM, overnight) and then untreated (-) or treated with Lipopolysaccharide (LPS) #14011 and Human Interferon-γ (hIFN-γ) #8901 (both at 100 ng/ml, 16 hr; +), using GBP5 (D3A5O) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
GBP5 (guanylate binding protein 5) is one of seven interferon-inducible GTPases in humans that have recently been shown to be involved in host defense against intracellular pathogens (1,2). Specifically, in response to intracellular bacteria or cell wall components, GBP5 acts as a tetramer to facilitate assembly of the NLRP3 inflammasome, leading to caspase-1 activation (2,3). In addition, GBP5 enables activation of the AIM2 inflammasome by promoting lysis of intracellular bacteria and release of pathogenic double-stranded DNA (4).
- Kim, B.H. et al. (2011) Science 332, 717-21.
- Kim, B.H. et al. (2012) Cell Host Microbe 12, 432-44.
- Shenoy, A.R. et al. (2012) Science 336, 481-5.
- Meunier, E. et al. (2015) Nat Immunol 16, 476-84.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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