Cell Signaling Technology

Product Pathways - CD Markers

DPP4/CD26 (D6D8K) Rabbit mAb #67138

sc-9153  

No. Size Price
67138S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
67138 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 90, 120 Rabbit IgG
IP 1:100
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

DPP4 (D6D8K) Rabbit mAb recognizes endogenous levels of total DPP4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu491 of human DPP4 protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of Caco-2 (positive, left) and HT-1080 (negative, right) cells using DPP4 (D6D8K) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from Lovo, Caco-2 and IGROV-1 cell lines using DPP4 (D6D8K) Rabbit mAb.

IP

IP

Immunoprecipitation of DPP4 protein from LoVo cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is DPP4 (D6D8K) Rabbit mAb. Western blot analysis was performed using DPP4 (D6D8K) Rabbit mAb.

Background

DDP4 (CD26) is a type II transmembrane glycoprotein expressed ubiquitously in most tissues and different cell types (1,2). The protein has a short cytoplasmic domain, transmembrane domain, a flexible stalk fragment and extracellular fragment (2). Both the catalytic peptide hydrolase domain and the beta-propeller ligand binding domain are located in the extracellular fragment (2). DDP4 is a multifunctional protein that exists in both a membrane bound form as well as an extracellular soluble form. As a peptidase, it removes N-terminal dipeptides sequentially from proteins with a proline or alanine as the penultimate P1 amino acid (3.4). DPP4 has been shown to cleave a wide range of substrates including GLP-1, BNP, substance P, etc. It is also involved in the regulation of related biological functions (5). In addition to it peptidase activity, DDP4 interacts with multiple important cell surface ligands, such as adenosine deaminase, fibronectin, and IGF2 receptor to influence processes like T cell activation, cell migration and proliferation (5). Several DDP4 inhibitors have been developed and their effects have been tested in the field of diabetes, cardiovascular disease and tumor immunity (2,5,6).

  1. Mentzel, S. et al. (1996) J Histochem Cytochem 44, 445-61.
  2. Röhrborn, D. et al. (2015) Front Immunol 6, 386.
  3. Hopsu-Havu, V.K. and Glenner, G.G. (1966) Histochemie 7, 197-201.
  4. Lone, A.M. et al. (2010) AAPS J 12, 483-91.
  5. Zhong, J. et al. (2015) J Diabetes Res 2015, 606031.
  6. Ohnuma, K. et al. (2015) Nat Immunol 16, 791-2.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications