Cell Signaling Technology

Product Pathways - Neuroscience

SV2A (D1L8S) Rabbit mAb #66724

sc-376234  

No. Size Price
66724S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
66724 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 80-100 Rabbit IgG
IP 1:50
IF-F 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-F=Immunofluorescence (Frozen),

Specificity / Sensitivity

SV2A (D1L8S) Rabbit mAb recognizes endogenous levels of total SV2A protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe516 of human SV2A protein.

IF-F

IF-F

Confocal immunofluorescent analysis of normal rat brain using SV2A (D1L8S) Rabbit mAb (green) and GFAP (GA5) Mouse mAb #3670 (red).

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse brain and NIH/3T3 cells using SV2A (D1L8S) Rabbit mAb.

Background

SV2s are a family of synaptic vesicle proteins expressed in both neurons and endocrine cells. SV2s function in the regulation of synaptic vesicle traffic, cytoplasmic Ca2+ levels in the nerve terminal during repetitive stimulation and the facilitation of synaptic transmission. There are three isoforms of SV2: SV2A, SV2B and SV2C. Each of these isoforms are structured similarly but expressed varyingly. SV2C, a minor isoform of SV2, expressed in a small subset of neurons located within the basal forebrain, midbrain and brainstem. SV2B, a major isoform of SV2 is expressed more abundantly, although rarely without the coexpression of SV2A. SV2A, the other major isoform of SV2 is the most widely expressed. SV2A is located in the presynaptic nerve terminals of almost every neuron throughout the nervous system. In addition, it is also located in most neuroendocrine secretory granules (1). SV2A has been identified as a critical protein for proper function of the central nervous system and has been linked to the physiopathology of epilepsy (2). In addition to the epileptic affects of this protein, mutations in it have also been seen to result in schizophrenia (3).

  1. Janz, R. et al. (1999) Neuron 24, 1003-16.
  2. van Vliet, E.A. et al. (2009) Epilepsia 50, 422-33.
  3. Mattheisen, M. et al. (2012) Schizophr Res 141, 262-5.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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