Product Pathways - Cell Cycle / Checkpoint
PARG (D4E6X) Rabbit mAb #66564
|66564S||100 µl ( 10 western blots )||￥3,250.00 现货查询||购买询价|
|66564||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation,
Specificity / Sensitivity
PARG (D4E6X) Rabbit mAb recognizes endogenous levels of total PARG protein. This antibody specifically recognizes PARG isoform 1 (UniProt #Q86W56-1) and does not recognize other PARG isoforms.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PARG protein.
Western blot analysis of extracts from HPB-ALL, HeLa, and COS-7 cells using PARG (D4E6X) Rabbit mAb.
Poly (ADP-ribose) glycohydrolase (PARG) is an enzyme that hydrolyzes poly (ADP-ribose) (PAR) formed by members of the PAR polymerase (PARP) enzyme family. Poly (ADP)-ribosylation is a post-translational modification that is catalyzed by PARP proteins. This modification involves polymerization of ADP-ribose from NAD+ to target proteins, such as histones and transcription factors, and plays a wide range of biological roles, including the response to DNA damage and transcriptional regulation (1,2). The mammalian PARG enzyme that catalyzes the removal of this modification exists as multiple isoforms. Longer PARG isoforms (100-110 kDa) shuttle between the nucleus and cytoplasm and are responsible for most of the PARG activity. The smaller (65 kDa) isoform resides in the cytoplasm (3-5). Research studies link altered PAR metabolism to inflammatory and autoimmune diseases, as well as neuronal degeneration (6-8). PARG inhibitors that increase PAR levels may sensitize cells to cancer treatments (e.g., cisplatin) and may help in the development of cancer therapies (9).
- Thomas, C. and Tulin, A.V. (2013) Mol Aspects Med 34, 1124-37.
- Li, N. and Chen, J. (2014) Mol Cells 37, 9-16.
- Meyer-Ficca, M.L. et al. (2004) Exp Cell Res 297, 521-32.
- Meyer-Ficca, M.L. et al. (2005) Int J Biochem Cell Biol 37, 920-6.
- Bonicalzi, M.E. et al. (2003) Biol Cell 95, 635-44.
- Cortes, U. et al. (2004) Mol Cell Biol 24, 7163-78.
- Masutani, M. et al. (2005) Cell Mol Life Sci 62, 769-83.
- Ying, W. and Swanson, R.A. (2000) Neuroreport 11, 1385-8.
- Fauzee, N.J. et al. (2010) Pathol Oncol Res 16, 469-78.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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For Research Use Only. Not For Use In Diagnostic Procedures.
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