Cell Signaling Technology

Product Pathways - Innate Immunity

cGAS (D1D3G) Rabbit mAb (Biotinylated) #66546

No. Size Price
66546S 100 µl ( 10 western blots ) ¥4,060.00 现货查询 购买询价 防伪查询
66546 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 62 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

cGAS (D1D3G) Rabbit mAb (Biotinylated) recognizes endogenous levels of total cGAS protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala19 of human cGAS protein.


This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated cGAS (D1D3G) Rabbit mAb #15102.

Western Blotting

Western Blotting

Western blot analysis of extracts from THP-1 and HeLa cell lines using cGAS (D1D3G) Rabbit mAb (Biotinylated).


Cyclic GMP-AMP synthase (cGAS, MB21D1) is an antiviral enzyme that produces the second messenger cyclic-GMP-AMP (cGAMP) in response to cytoplasmic DNA (1,2). The cGAS protein acts as a cytosolic DNA sensor that binds DNA and produces the cGAMP second messenger from ATP and GTP (1,2). cGAMP binds to and activates STING, a transmembrane adaptor protein that is a critical component of the cellular innate immune response to pathogenic cytoplasmic DNA (1-4). STING is ubiquitously expressed and found predominantly in the ER (3). Following activation, STING translocates with TBK1 to perinuclear endosomes (5). The TBK1 kinase phosphorylates and activates interferon regulatory factors (IRFs) and NF-κB, which leads to the induction of type I interferon and other immune response genes (3-5).

  1. Sun, L. et al. (2013) Science 339, 786-91.
  2. Wu, J. et al. (2013) Science 339, 826-30.
  3. Ishikawa, H. and Barber, G.N. (2008) Nature 455, 674-8.
  4. Zhong, B. et al. (2008) Immunity 29, 538-50.
  5. Ishikawa, H. et al. (2009) Nature 461, 788-92.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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