Cell Signaling Technology

Product Pathways - Adhesion

Olfm4 Antibody (Mouse Specific) #66479

No. Size Price
66479S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
66479 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse, Endogenous 85-90, 70 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Olfm4 Antibody (Mouse Specific) recognizes endogenous levels of total mouse Olfm4 protein. This antibody does not cross-react with human OLFM4.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala85 of mouse Olfm4 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse small intestine and mouse colon, using Olfm4 (Mouse Specific) Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As reported (10), mouse small intestine is positive and mouse colon is negative for Olfm4 protein expression.

Background

Olfactomedin-4 (OLFM4, hGC-1) is a member of the Olfactomedin family, a small group of extracellular proteins defined by the presence of a conserved "Olfactomedin domain" that is thought to facilitate protein-protein interactions (1). OLFM4 is a secreted glycoprotein, which forms disulfide bond-mediated oligomers, and is thought to mediate cell adhesion through its interactions with extracellular matrix proteins such as lectins (2). Human OLFM4 was first cloned from myeloid cells (3) and is expressed in a distinct subset of neutrophils, though the functional significance of this differential expression pattern remains unclear (4). Among normal tissues, the expression of OLFM4 protein is most abundant in intestinal crypts (5), where it has garnered attention as a possible marker of intestinal stem cells (6). Notably, OLFM4 expression is markedly increased in several tumor types, including colorectal, gastric, pancreas, lung, and breast (reviewed in [1]). Furthermore, research studies show that the expression levels of OLFM4 vary in relation to the severity and/or differentiation status of multiple tumor types (1, 6-8), leading to the suggestion that OLFM4 may have utility as a prognostic marker in some cancer patients (9).

Genetic analyses of murine LGR5 stem cells identified Olfm4 as a robust intestinal stem cell marker, but unlike its human homolog, mouse Olfm4 expression is not detected in colonic epithelial crypts (10).

  1. Yu, L. et al. (2011) Neoplasma 58, 9-13.
  2. Liu, W. et al. (2006) Exp Cell Res 312, 1785-97.
  3. Zhang, J. et al. (2002) Gene 283, 83-93.
  4. Welin, A. et al. (2013) PLoS One 8, e69575.
  5. van der Flier, L.G. et al. (2009) Gastroenterology 137, 15-7.
  6. Liu, W. et al. (2007) Histopathology 51, 157-65.
  7. Liu, W. et al. (2008) Clin Cancer Res 14, 1041-9.
  8. Besson, D. et al. (2011) Mol Cell Proteomics 10, M111.009712.
  9. Seko, N. et al. (2010) Exp Ther Med 1, 73-8.
  10. van der Flier, L.G. et al. (2009) Cell 136, 903-12.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications