Cell Signaling Technology

Product Pathways - Development

Lunatic Fringe (D6V2V) Rabbit mAb #66472

O-fucosylpeptide 3-beta-N-acetyleglycosaminyltransferase  

No. Size Price
66472S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
66472 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 42 Rabbit IgG
IP 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Lunatic Fringe (D6V2V) Rabbit mAb recognizes endogenous levels of total Lunatic Fringe protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu74 of human Lunatic Fringe protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Lunatic Fringe (D6V2V) Rabbit mAb.

Background

Lunatic Fringe (Beta-1,3-N-acetylglucosaminyltransferase, LFNG) is a single-pass type II Golgi membrane glycosyltransferase that catalyzes the elongation of O-linked fucose residues on EGF-like repeats of Notch signaling molecules. Fucosylation of EGF-like repeats serves to fine-tune Notch ligand-receptor interactions, thereby modulating downstream Notch pathway activity (1). Studies in genetic mouse models have shown that Lunatic Fringe-mediated Notch regulation is critical for somite patterning during vertebrate embryogenesis (2-4). Consistent with this, loss-of-function mutations in human LFNG are associated with spondylocostal dysostoses, a heritable skeletal growth disorder characterized by malformations of the spinal column and thoracic structures (5). Lunatic Fringe continues to modulate Notch signaling postnatally (6), and is implicated as a putative tumor suppressor in multiple Notch-related cancers (7, 8).

  1. Shimizu, K. et al. (2001) J Biol Chem 276, 25753-8.
  2. Evrard, Y.A. et al. (1998) Nature 394, 377-81.
  3. Zhang, N. and Gridley, T. (1998) Nature 394, 374-7.
  4. Serth, K. et al. (2015) PLoS One 10, e0123776.
  5. Sparrow, D.B. et al. (2006) Am J Hum Genet 78, 28-37.
  6. Mukherjee, S. et al. (2014) J Immunol 192, 996-1003.
  7. Zhang, S. et al. (2014) Neoplasia 16, 158-67.
  8. Zhang, S. et al. (2015) Oncogene , .

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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