Cell Signaling Technology

Product Pathways - Metabolism

PHGDH (D8F3O) Rabbit mAb #66350

No. Size Price
66350S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
66350 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 57 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

PHGDH (D8F3O) Rabbit mAb recognizes endogenous levels of total PHGDH protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val304 of human PHGDH protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PHGDH (D8F3O) Rabbit mAb.

Background

Mammalian cells synthesize serine de novo by diverting a portion of the glycolytic intermediate 3-phosphoglycerate into the phosphorylated pathway of serine synthesis. This shift supports anabolism by providing precursors for the biosynthesis of proteins, nucleotides, creatine, porphyrins, phospholipids, and glutathione. Phosphoglycerate dehydrogenase (PHGDH) catalyzes the first step in the serine biosynthesis pathway by converting 3-phosphoglycerate into phosphohydroxy pyruvate (1).

Research studies demonstrate that an increase in serine biosynthesis supports growth and proliferation of cancer cells (2-4), which is supported by amplification and overexpression of PHGDH in a subset of melanoma and breast cancers (5,6). Suppression of PHGDH expression in cell lines with elevated PHGDH levels causes a strong decrease in cell proliferation and inhibits tumor growth in vivo (5). Additional evidence suggests that PHGDH interacts with and stabilizes FoxM1, which promotes the proliferation, invasion, and tumorigenicity of glioma cells (7).

  1. Locasale, J.W. (2013) Nat Rev Cancer 13, 572-83.
  2. Amelio, I. et al. (2013) Oncogene, [Epub ahead of print].
  3. Ma, L. et al. (2013) Cell 152, 599-611.
  4. Maddocks, O.D. et al. (2013) Nature 493, 542-6.
  5. Possemato, R. et al. (2011) Nature 476, 346-50.
  6. Locasale, J.W. et al. (2011) Nat Genet 43, 869-74.
  7. Liu, J. et al. (2013) J Neurooncol 111, 245-55.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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