Cell Signaling Technology

Product Pathways - Apoptosis

Annexin V-FITC Early Apoptosis Detection Kit #6592


No. Size Price
6592S 1 Kit ( 100 assays ) ¥5,301.00 现货查询 购买询价
Kit Includes Quantity Applications Reactivity Homology† MW (kDa) Isotype
10X Annexin V Binding Buffer 1.5 ml
Propidium Iodide (PI) Solution 1.3 ml
Annexin V-FITC Conjugate 100 µl


The Annexin V-FITC Early Apoptosis Detection Kit enables researchers to identify early apoptotic cells within a cell population. Annexin V-FITC conjugated protein binds to cell surfaces expressing phosphatidylserine, an early apotosis marker. Cells stained with propidium iodide (PI), a non-cell-permeable DNA dye, indicate necrotic cells. Cells stained with both PI and annexin V-FITC demonstrate later stage apoptosis and early necrosis. This kit provides enough reagent to perform 100 assays, based on a 250 μl assay volume.

Annexin V-FITC Early Apoptosis Detection Kit使研究者能够从细胞群中鉴定出早期凋亡细胞。Annexin V-FITC结合蛋白能结合到细胞表面表达丝氨酸磷脂(一种早期凋亡标志)。细胞用碘化丙啶(PI,一种非细胞可透过性DNA染料)染色能指示出坏死的细胞。细胞同时用PI和annexin V-FITC染色能指示出较晚阶段的凋亡和早期坏死。本试剂盒提供的试剂足够进行100次分析,250 μl/次。



Confocal immunofluorescent analysis of live Jurkat cells, untreated (left) or camptothecin-treated (right), using Annexin V-FITC Conjugate (green). Red = Propidium Iodide (fluorescent DNA dye). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析活的Jurkat细胞,未处理(左图)或用喜树碱处理(右图),使用的抗体是:Annexin V-FITC结合物(绿色)。红色=碘化丙啶(DNA荧光染料)。蓝色假色= DRAQ5® #4084(DNA荧光染料)。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells untreated (left) or treated with Staurosporine #9953 (1 μM, 2 hr; right) using Annexin V-FITC Early Apoptosis Detection Kit.流式细胞检测分析Jurkat细胞,未处理(左图)或用Staurosporine #9953 (1 μM, 2小时; 右图)处理,使用Annexin V-FITC Early Apoptosis Detection Kit。


Annexin V belongs to the annexin family of proteins consisting of over 160 members. All annexin family members share the same charactersistic of Ca2+-dependent binding to negatively charged phospholipid surfaces (1). During early apoptosis, phosphatidylserine (PS) is translocated from the cytosolic side of the plasma membrane to the cellular surface. This translocation exposes PS to the extracellular environment with the plasma membrane left intact (2). Annexin V protein demonstrates high affinity, specificity, and sensitivity for PS and can be used as a marker of early apoptosis. In order to rule out "leaky" necrotic cells, annexin V must be used in tandem with reagents that determine the integrity of the cell membrane, such as propidium iodide (2).

钙磷脂结合蛋白Ⅴ(Annexin V)属于Annexin蛋白质家族,该家族成员超过160个。所有Annexin家族成员都有相同的钙离子依赖结合到负电荷的磷脂表面的特性(1)。在早期凋亡过程中,丝氨酸磷脂(PS)从质膜的细胞溶质边转移到细胞的表面。这种迁移能够在质膜原封不动的情况下使PS暴露到细胞外环境中(2)。Annexin V蛋白对PS有高亲和性,特异性和敏感性,并且可以作为一种早期凋亡的标志。为了排除“遗漏的”坏死细胞,Annexin V必须与能够检测细胞膜完整性的试剂(如碘化丙啶)联用(2)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

The Annexin V-FITC conjugate is a product manufactured for Cell Signaling Technology, Inc. by TAU Technologies BV.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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