Cell Signaling Technology

Product Pathways - Adhesion

Mesothelin (D4X7M) Rabbit mAb #65367

Cleaved mesothelin   MSLN  

No. Size Price
65367S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
65367 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 46-48, 70 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Mesothelin (D4X7M) Rabbit mAb recognizes endogenous levels of total mesothelin protein. The antibody recognizes both uncleaved (full-length) and cleaved forms of mesothelin, but does not detect the cleaved fragment corresponding to megakaryocyte-potentiating factor (MPF).

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly577 of human mesothelin protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Mesothelin (D4X7M) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from OVCAR8 cells, untreated (-) or PNGase F-treated (+), using Mesothelin (D4X7M) Rabbit mAb. Results demonstrate change in electrophoretic mobility of mesothelin following deglycosylation by PNGase F.

IP

IP

Immunoprecipitation of mesothelin from OVCAR8 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Mesothelin (D4X7M) Rabbit mAb. Western blot analysis was performed using Mesothelin (D4X7M) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody.

Background

The MSLN gene encodes a 69 kDa precursor protein that is proteolytically cleaved to yield Megakaryocyte Potentiating Factor (MPF) and a GPI-anchored membrane protein termed mesothelin (1). Expression of (cleaved) mesothelin is largely confined to mesothelial cells of normal pleura, pericardium, and peritoneum, but has been reported to be overexpressed in some cancers, including mesothelioma, and some pancreatic and ovarian adenocarcinomas (1,2). Although suggested to be involved in cell adhesion, the physiological functions of mesothelin have not been determined. It is known, however, that mesothelin can be shed from the cell surface following cleavage by TNF-α converting enzyme. Research studies show that serum levels of mesothelin are markedly increased in patients with mesothelioma and ovarian cancer (1), suggesting that serum mesothelin levels may have utility as a cancer biomarker (1-3).

  1. Pastan, I. and Hassan, R. (2014) Cancer Res 74, 2907-12.
  2. Villena-Vargas, J. and Adusumilli, P.S. (2012) Ann Cardiothorac Surg 1, 466-71.
  3. Hassan, R. et al. (2004) Clin Cancer Res 10, 3937-42.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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