Cell Signaling Technology

Product Pathways - Neuroscience

Endophilin-1 (E1E6Q) Rabbit mAb #65169

No. Size Price
65169S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
65169 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 41 Rabbit IgG
IP 1:50
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Endophilin-1 (E1E6Q) Rabbit mAb recognizes endogenous levels of total endophilin-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly268 of human endophilin-1 protein.



Confocal immunofluorescent analysis of HSG (left) or MCF7 (right) cells using Endophilin-1 (E1E6Q) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines and tissues using Endophilin-1 (E1E6Q) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).



Immunoprecipitation of endophilin-1 from mouse brain extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Endophilin-1 (E1E6Q) Rabbit mAb (lane 3). Western blot analysis was performed using Endophilin-1 (E1E6Q) Rabbit mAb.


Endophilin proteins are part of a large family of Bin/Amphiphysin/Rvs (BAR) domain proteins that are involved in cell membrane remodeling (1). The endophilins are encoded by five genes, which produce endophilin A 1-3 and B 1-2 (2). Endophilins are involved in many cellular mechanisms, such as synaptic vesicle recycling, receptor trafficking, and membrane remodeling processes (2). Research studies indicate that endophilin 1 (endophilin A1, SH3GL2) can induce different membrane shapes (3) and participate in the morphogenesis of dendritic spines (4). Endophilin 1 is also involved in regulating blood brain barrier permeability via the EGFR-JNK pathway (5).

  1. Safari, F. and Suetsugu, S. (2012) Membranes (Basel) 2, 91-117.
  2. Kjaerulff, O. et al. (2011) Cell Biochem Biophys 60, 137-54.
  3. Ambroso, M.R. et al. (2014) Proc Natl Acad Sci U S A 111, 6982-7.
  4. Yang, Y. et al. (2015) Cell Res 25, 496-516.
  5. Chen, L. et al. (2015) Brain Res 1606, 44-53.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :