Cell Signaling Technology

Product Pathways - Neuroscience

Endophilin-1 (E1E6Q) Rabbit mAb #65169

No. Size Price
65169S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
65169 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 41 Rabbit IgG
IP 1:50
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Endophilin-1 (E1E6Q) Rabbit mAb recognizes endogenous levels of total endophilin-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly268 of human endophilin-1 protein.



Confocal immunofluorescent analysis of HSG (left) or MCF7 (right) cells using Endophilin-1 (E1E6Q) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines and tissues using Endophilin-1 (E1E6Q) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).



Immunoprecipitation of endophilin-1 from mouse brain extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Endophilin-1 (E1E6Q) Rabbit mAb (lane 3). Western blot analysis was performed using Endophilin-1 (E1E6Q) Rabbit mAb.


Endophilin proteins are part of a large family of Bin/Amphiphysin/Rvs (BAR) domain proteins that are involved in cell membrane remodeling (1). The endophilins are encoded by five genes, which produce endophilin A 1-3 and B 1-2 (2). Endophilins are involved in many cellular mechanisms, such as synaptic vesicle recycling, receptor trafficking, and membrane remodeling processes (2). Research studies indicate that endophilin 1 (endophilin A1, SH3GL2) can induce different membrane shapes (3) and participate in the morphogenesis of dendritic spines (4). Endophilin 1 is also involved in regulating blood brain barrier permeability via the EGFR-JNK pathway (5).

  1. Safari, F. and Suetsugu, S. (2012) Membranes (Basel) 2, 91-117.
  2. Kjaerulff, O. et al. (2011) Cell Biochem Biophys 60, 137-54.
  3. Ambroso, M.R. et al. (2014) Proc Natl Acad Sci U S A 111, 6982-7.
  4. Yang, Y. et al. (2015) Cell Res 25, 496-516.
  5. Chen, L. et al. (2015) Brain Res 1606, 44-53.

Application References

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