Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

CHD7 (D3F5) Rabbit mAb #6505


No. Size Price
6505S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
6505 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 336 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

CHD7 (D3F5) Rabbit mAb recognizes endogenous levels of total CHD7 protein. This antibody also cross-reacts with proteins of unknown origin at 50-55 kDa.

CHD7 (D3F5) Rabbit mAb兔单抗能够检测内源性的CHD8总蛋白水平。该抗体也与未知来源50-55 kDa大小蛋白发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the amino terminus of human CHD7 protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CHD7 (D3F5) Rabbit mAb.

使用CHD7 (D3F5) Rabbit mAb兔单抗,免疫印迹(Western blot)分析不同细胞中CHD7蛋白水平。


CHD7 belongs to the chromodomain helicase DNA-binding (CHD) family of ATP-dependent chromatin remodeling proteins (1). The CHD family of proteins has been shown to play an important role in regulating gene expression by altering the chromatin structure at target genes (1,2). The nine members of the CHD family are characterized by the presence of two tandem chromodomains in the N-terminal region and an SNF2-like ATPase domain near the central region of the protein (2-4). The CHD proteins can be further divided into three subgroups based on the presence of additional conserved functional domains. CHD7 belongs to the third subgroup of CHD proteins together with CHD6, 8, and 9, which are distinguished by the presence of three conserved region (CR) domains, a switching-defective protein 3, adaptor 2, nuclear receptor co-repressor, transcription factor IIB (SANT) like domain, two brahma and kismet (BRK) domains, and a DNA binding domain (2). CHD7 regulates embryonic stem cell (ESC) specific gene expression together with ESC master regulators Oct-4, Sox2 and nanog, and is necessary for neural stem cell development and formation of the neural crest (5-7). Research studies have shown that CHD7 mutations are frequently found in patients with CHARGE syndrome (coloboma of the eye, heart defects, atresia of the choanae, retardation of growth/development, genital/urinary abnormalities, and ear abnormalities and deafness) (8).

CHD7属于ATP依赖的染色质重塑蛋白的chromodomain helicase DNA-binding (CHD)家族 (1)。研究证明CHD蛋白家族通过改变染色质结构从而在调节基因表达中起到重要作用(1,2)。九种CHD家族成员都通过在N-端区域有两个串联的chromodomain和在蛋白的中间区域一个SNF2-like ATPase结构域存在为特征 (2-4)。CHD蛋白能被进一步划分为三个亚群,这是基于另外保守功能结构域的存在。CHD7与CHD6、8、9一起属于CHD蛋白的第三个亚群,这些蛋白通过三个conserved region (CR)结构域、一个switching-defective protein 3、adaptor 2、nuclear receptor co-repressor、transcription factor IIB (SANT) like domain、两个brahma and kismet (BRK) domains和一个DNA binding domain的存在而区别 (2)。CHD7蛋白与ESC主要调节因子Oct-4、Sox2和nanog一起调节胚胎肝细胞 (embryonic stem cell,ESC)特异性基因,并且对于神经干细胞法乐和神经嵴的形成是必须的(5-7)。研究证明CHD7突变频繁的被发现在CHARGE综合征(眼睛缺损、心脏缺陷、后鼻孔的闭锁、增长/发展的阻滞、生殖器/尿异常和耳畸形和耳聋) (8)。

  1. Hargreaves, D.C. and Crabtree, G.R. (2011) Cell Res 21, 396-420.
  2. Marfella, C.G. and Imbalzano, A.N. (2007) Mutat Res 618, 30-40.
  3. Delmas, V. et al. (1993) Proc Natl Acad Sci U S A 90, 2414-8.
  4. Woodage, T. et al. (1997) Proc Natl Acad Sci U S A 94, 11472-7.
  5. Schnetz, M.P. et al. (2010) PLoS Genet 6, e1001023.
  6. Engelen, E. et al. (2011) Nat Genet 43, 607-11.
  7. Bajpai, R. et al. (2010) Nature 463, 958-62.
  8. Vissers, L.E. et al. (2004) Nat Genet 36, 955-7.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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