Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

EphA7 (D1C3K) Rabbit mAb #64801

No. Size Price
64801S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
64801 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 130 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

EphA7 (D1C3K) Rabbit mAb recognizes endogenous levels of total EphA7 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu914 of human EphA7 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from ACHN, LNCaP, and RD cell lines using EphA7 (D1C3K) Rabbit mAb.

IP

IP

Immunoprecipitation of EphA7 protein from ACHN cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is EphA7 (D1C3K) Rabbit mAb. Western blot analysis was performed using EphA7 (D1C3K) Rabbit mAb.

Background

The Eph receptors are the largest known family of receptor tyrosine kinases (RTKs). They can be divided into two groups based on sequence similarity and on their preference for a subset of ligands. While EphA receptors bind to a glycosylphosphatidylinositol-anchored ephrin A ligand, EphB receptors bind to ephrin B proteins that have a transmembrane and cytoplasmic domain (1,2). Research studies have shown that Eph receptors and ligands may be involved in many diseases including cancer (3). Both ephrin A and B ligands have dual functions. As RTK ligands, ephrins stimulate the kinase activity of Eph receptors and activate signaling pathways in receptor-expressing cells. The ephrin extracellular domain is sufficient for this function as long as it is clustered (4). The second function of ephrins has been described as "reverse signaling", whereby the cytoplasmic domain becomes tyrosine phosphorylated, allowing interactions with other proteins that may activate signaling pathways in the ligand-expressing cells (5).

The EphA7 receptor preferentially binds ephrin-A5 as a ligand. This ligand-receptor interaction stimulates EphA7 signaling and induces apoptotic cell death through TNFR1 and caspase-8 pathway (6,7). EphA7 plays a critical role in organ development during neural tube closure, cortical dendritic development and spine maturation as well as urine tract insertion (8-10). Secreted EphA7 has been shown to promote somatic cell reprogramming through ERK activity reduction (11). Silencing of the secreted form of EphA7 is associated with germinal center B cell lymhomas. The secreted form of EphA7 has been proposed as a soluble tumor suppresor in lymphoma (12-14).

  1. Wilkinson, D.G. (2000) Int Rev Cytol 196, 177-244.
  2. Klein, R. (2001) Curr Opin Cell Biol 13, 196-203.
  3. Dodelet, V.C. and Pasquale, E.B. (2000) Oncogene 19, 5614-9.
  4. Holder, N. and Klein, R. (1999) Development 126, 2033-44.
  5. Brückner, K. et al. (1997) Science 275, 1640-3.
  6. Lee, H. et al. (2013) Mol Cells 35, 450-5.
  7. Lee, H. et al. (2015) Mol Cells 38, 349-55.
  8. Lee, J. et al. (2013) Dev Growth Differ 55, 341-9.
  9. Clifford, M.A. et al. (2014) Proc Natl Acad Sci U S A 111, 4994-9.
  10. Weiss, A.C. et al. (2014) Development 141, 3420-30.
  11. Lee, J. et al. (2015) Stem Cell Reports 5, 480-9.
  12. Oricchio, E. and Wendel, H.G. (2012) Cell Cycle 11, 1076-80.
  13. Dawson, D.W. et al. (2007) Oncogene 26, 4243-52.
  14. Oricchio, E. et al. (2011) Cell 147, 554-64.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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