Cell Signaling Technology

Product Pathways - Nuclear Receptor Signaling

COUP-TFII (D16C4) Rabbit mAb #6434

No. Size Price
6434S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价
6434 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 47 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

COUP-TFII (D16C4) Rabbit mAb detects endogenous levels of total COUP-TFII protein. The antibody does not cross-react with COUP-TFI protein.COUP-TFII (D16C4) Rabbit mAb能够检测内源性水平的总COUP-TFII蛋白。该抗体不与COUP-TFI蛋白发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln158 of human COUP-TFII protein.该单克隆抗体通过用合成肽免疫动物制备,该合成肽是人COUP-TFII蛋白谷氨酰胺(158位)附近的残基。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using COUP-TFII (D16C4) Rabbit mAb.Western blot方法检测多个细胞系提取物,使用的抗体为COUP-TFII (D16C4) Rabbit mAb.


Chicken ovalbumin upstream promoter transcription factor (COUP-TF) belongs to the NR2 subfamily of the nuclear hormone receptor family (1). COUP-TFI and COUP-TFII are two of the well-characterized members in the NR2 subfamily. These two members are highly conserved in their two zinc-finger DNA binding domains (DBD) and the ligand binding domain (LBD), and function as repressors or activators of downstream target genes to regulate different biological processes (1-3). COUP-TFI and II bind to 5'-AGGTCA-3' motif palindromes, either directly or indirectly, through heterodimer formation with other proteins (e.g. RXRs) to regulate downstream target gene expression (4,5). COUP-TFI is involved in neuronal development, tissue patterning, and differentiation (6-8). COUP-TFII has been shown to be involved in angiogenesis, glucose homeostasis, and mesenchymal cell commitment (9-12).鸡卵清蛋白上游启动子转录因子(COUP-TF)属于核激素受体家族的NR2亚科(1)。 COUP-TFI和COUP-TFII是两个良好的特征性NR2亚家族成员。这两个成员在其两个锌指DNA结合域和配体结合域(LBD)是高度保守的,可作为下游靶基因的阻遏物或激活剂的功能,来调节不同的生物过程(1-3)。COUP-TFI和II直接或间接地结合5'-AGGTCA-3'模体回文,通过与其他蛋白质(如RXR)形成异源二聚体来调节下游靶基因的表达(4,5)。COUP-TFI参与神经元的发育、组织发生和分化(6-8)。 已证明COUP-TFII参与血管生成、葡萄糖稳态和间充质细胞的定型(9-12)。

  1. Tsai, S.Y. and Tsai, M.J. (1997) Endocr Rev 18, 229-40.
  2. Park, J.I. et al. (2003) Keio J Med 52, 174-81.
  3. Lin, F.J. et al. (2011) Endocr Rev 32, 404-21.
  4. Cooney, A.J. et al. (1992) Mol Cell Biol 12, 4153-63.
  5. Montemayor, C. et al. (2010) PLoS One 5, e8910.
  6. Zhou, C. et al. (2001) Genes Dev 15, 2054-9.
  7. Alfano, C. et al. (2011) Development 138, 4685-97.
  8. Faedo, A. et al. (2008) Cereb Cortex 18, 2117-31.
  9. Li, L. et al. (2009) Cell Metab 9, 77-87.
  10. Pereira, F.A. et al. (1999) Genes Dev 13, 1037-49.
  11. Qin, J. et al. (2010) Cancer Res 70, 8812-21.
  12. Xie, X. et al. (2011) Proc Natl Acad Sci U S A 108, 14843-8.

Application References

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