Product Pathways - Neuroscience
ARALAR/AGC1 (D5I6I) Rabbit mAb #64169
|64169S||100 µl ( 10 western blots )||￥3,100.00 现货查询||购买询价|
|64169||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation,
Specificity / Sensitivity
ARALAR/AGC1 (D5I6I) Rabbit mAb recognizes endogenous levels of total ARALAR/AGC1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg309 of human ARALAR/AGC1 protein.
Immunoprecipitation of ARALAR/AGC1 from mouse brain extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is ARALAR/AGC1 (D5I6I) Rabbit mAb (lane 3). Western blot analysis was performed using ARALAR/AGC1 (D5I6I) Rabbit mAb.
Mitochondrial carriers are integral proteins of the mitochondrial inner membrane that transport metabolites, nucleotides, and co-factors between the cytosol and the mitochondria (1). The calcium-binding mitochondrial carrier protein ARALAR (SLC25A12, AGC1) is an aspartate-glutamate exchange protein responsible for transporting mitochondrial aspartate across the mitochondrial inner membrane in exchange for cytosolic glutamate (2,3). ARALAR and other proteins of the aspartate-glutamate carrier (AGC) group are required for the transfer of mitochondrial aspartate to the cytosol, a key step in urea synthesis (4). Research studies using ARALAR-knockout mice indicate that ARALAR plays an important role in proper CNS myelination. Mice lacking ARALAR suffer from hypomyelination as a result of a lack of oligodendrocyte maturation caused by decreased brain N-acetylaspartate levels (5). Mutation of the corresponding SLC25A12 gene can result in global cerebral hypomyelination and severe psychomotor retardation, caused by deficient ARALAR activity and limited mitochondrial aspartate efflux (6).
- Walker, J.E. and Runswick, M.J. (1993) J Bioenerg Biomembr 25, 435-46.
- del Arco, A. and Satrústegui, J. (1998) J Biol Chem 273, 23327-34.
- Palmieri, L. et al. (2001) EMBO J 20, 5060-9.
- Satrústegui, J. et al. (2007) Physiol Rev 87, 29-67.
- Ramos, M. et al. (2011) J Neurosci Res 89, 2008-17.
- Wibom, R. et al. (2009) N Engl J Med 361, 489-95.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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