Product Pathways - Protein Stability
UBE2G2 (D8Z4G) Rabbit mAb #63182
|63182S||100 µl ( 10 western blots )||￥3,100.00 现货查询||购买询价|
|63182||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting,
Species predicted to react based on 100% sequence homology: Bovine, Pig,
Specificity / Sensitivity
UBE2G2 (D8Z4G) Rabbit mAb recognizes endogenous levels of total UBE2G2 protein. This antibody does not cross-react with UBE2G1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human UBE2G2 protein.
Ubiquitin (Ub) is a conserved polypeptide that is covalently linked to many cellular proteins through the process of ubiquitination, which targets proteins for degradation by the 26S proteasome. Three enzymatic components are involved in the protein ubiquitination cascade. Ubiquitin is first activated by forming a thioester complex with an E1 ubiquitin-activating enzyme. Activated ubiquitin is subsequently transferred to an E2 ubiquitin-carrier protein, and then from the E2 to an E3 ubiquitin ligase for final delivery to the ε-amino group of the target protein lysine residue (1-3).
The ubiquitin-conjugating enzyme E2 G2 (UBE2G2, UBC7) is a ubiquitously expressed E2 enzyme and critical component of the endoplasmic reticulum-associated degradation pathway (ERAD) (4). Research studies demonstrate that UBE2G2 forms homodimers and preassembles K48-linked poly-Ub chains at its active site (5-8). The association of Ub-charged UBE2G2 molecules with the ER-resident E3 ligase AMFR (gp78) is required for Ub chain transfer and efficient removal of misfolded or aggregated proteins through the ERAD pathway (9,10).
- Ciechanover, A. (1998) EMBO J 17, 7151-60.
- Hochstrasser, M. (2000) Nat Cell Biol 2, E153-7.
- Hochstrasser, M. (2000) Science 289, 563-4.
- Katsanis, N. and Fisher, E.M. (1998) Genomics 51, 128-31.
- Bazirgan, O.A. and Hampton, R.Y. (2008) J Biol Chem 283, 12797-810.
- Ravid, T. and Hochstrasser, M. (2007) Nat Cell Biol 9, 422-7.
- Liu, W. et al. (2014) EMBO J 33, 46-61.
- Li, W. et al. (2007) Nature 446, 333-7.
- Li, W. et al. (2009) Proc Natl Acad Sci U S A 106, 3722-7.
- Chen, B. et al. (2006) Proc Natl Acad Sci U S A 103, 341-6.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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